Difference between revisions of "EpsB"

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* '''Description:''' extracellular polysaccharide synthesis, putative protein tyrosine kinase <br/><br/>
+
* '''Description:''' extracellular polysaccharide synthesis, protein tyrosine kinase <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || unknown
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|style="background:#ABCDEF;" align="center"| '''Product''' || protein tyrosine kinase
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Function''' || [[biofilm formation]]  
 
|style="background:#ABCDEF;" align="center"|'''Function''' || [[biofilm formation]]  
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= [[Categories]] containing this gene/protein =
 
= [[Categories]] containing this gene/protein =
 
{{SubtiWiki category|[[protein modification]]}},
 
{{SubtiWiki category|[[protein modification]]}},
{{SubtiWiki category|[[biofilm formation]]}}
+
{{SubtiWiki category|[[biofilm formation]]}},
 +
{{SubtiWiki category|[[phosphoproteins]]}}
  
 
= This gene is a member of the following [[regulons]] =
 
= This gene is a member of the following [[regulons]] =
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* '''[[Domains]]:'''  
 
* '''[[Domains]]:'''  
  
* '''Modification:'''
+
* '''Modification:''' autophosphorylated on Tyr-225 and Tyr-227 {{PubMed|25085422}}
  
 
* '''[[Cofactors]]:'''
 
* '''[[Cofactors]]:'''

Revision as of 09:30, 5 August 2014

  • Description: extracellular polysaccharide synthesis, protein tyrosine kinase

Gene name epsB
Synonyms yveL
Essential no
Product protein tyrosine kinase
Function biofilm formation
Gene expression levels in SubtiExpress: epsB
Interactions involving this protein in SubtInteract: EpsB
Regulation of this protein in SubtiPathways:
epsB
MW, pI 24 kDa, 9.918
Gene length, protein length 681 bp, 227 aa
Immediate neighbours epsC, epsA
Sequences Protein DNA DNA_with_flanks
Genetic context
YveL context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
EpsB expression.png















Categories containing this gene/protein

protein modification, biofilm formation, phosphoproteins

This gene is a member of the following regulons

AbrB regulon, RemA regulon, SinR regulon

The gene

Basic information

  • Locus tag: BSU34360

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate (according to Swiss-Prot)
  • Paralogous protein(s): PtkA

Extended information on the protein

  • Kinetic information:
  • Modification: autophosphorylated on Tyr-225 and Tyr-227 PubMed
  • Effectors of protein activity:

Database entries

  • Structure: 2VED (CapB, the homolog in Staphylococcus aureus) PubMed
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH) PubMed, available in Jörg Stülke's lab
  • Antibody:

Labs working on this gene/protein

Richard Losick, Harvard Univ., Cambridge, USA homepage

Your additional remarks

References

Reviews

Joseph D Chao, Dennis Wong, Yossef Av-Gay
Microbial protein-tyrosine kinases.
J Biol Chem: 2014, 289(14);9463-72
[PubMed:24554699] [WorldCat.org] [DOI] (I p)

Massimiliano Marvasi, Pieter T Visscher, Lilliam Casillas Martinez
Exopolymeric substances (EPS) from Bacillus subtilis: polymers and genes encoding their synthesis.
FEMS Microbiol Lett: 2010, 313(1);1-9
[PubMed:20735481] [WorldCat.org] [DOI] (I p)


Original publications