Difference between revisions of "EpsH"

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{{SubtiWiki regulon|[[AbrB regulon]]}},
 
{{SubtiWiki regulon|[[AbrB regulon]]}},
 
{{SubtiWiki regulon|[[EAR riboswitch]]}},
 
{{SubtiWiki regulon|[[EAR riboswitch]]}},
 +
{{SubtiWiki regulon|[[RemA regulon]]}},
 
{{SubtiWiki regulon|[[SinR regulon]]}}
 
{{SubtiWiki regulon|[[SinR regulon]]}}
  
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* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
−
** [[SinR]]: transcription repression {{PubMed|15661000}}
+
** [[SinR]]: transcription anti-activation (prevents binding of [[RemA]]) {{PubMed|23646920}}
 +
** [[RemA]]: transcription activation {{PubMed|23646920}}
 
** [[AbrB]]: transcription repression {{PubMed|20817675}}
 
** [[AbrB]]: transcription repression {{PubMed|20817675}}
  
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<pubmed>20374491 20230605 </pubmed>
 
<pubmed>20374491 20230605 </pubmed>
 
===Other original publications===
 
===Other original publications===
−
<pubmed>23012477 15661000,16430695,18047568,11572999 ,15083514 18647168 20817675 21856853 21815947</pubmed>
+
<pubmed>23012477 15661000,16430695,18047568,11572999 ,15083514 18647168 20817675 21856853 21815947 23646920</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 15:25, 24 May 2013

  • Description: extracellular polysaccharide synthesis

Gene name epsH
Synonyms yveR
Essential no
Product unknown
Function biofilm formation
Gene expression levels in SubtiExpress: epsH
Regulation of this protein in SubtiPathways:
Biofilm
MW, pI 39 kDa, 5.788
Gene length, protein length 1032 bp, 344 aa
Immediate neighbours epsI, epsG
Sequences Protein DNA DNA_with_flanks
Genetic context
YveR context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
EpsH expression.png















Categories containing this gene/protein

biofilm formation

This gene is a member of the following regulons

AbrB regulon, EAR riboswitch, RemA regulon, SinR regulon

The gene

Basic information

  • Locus tag: BSU34300

Phenotypes of a mutant

  • altered cell death pattern in colonies PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: glycosyltransferase 2 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Richard Losick, Harvard Univ., Cambridge, USA homepage

Your additional remarks

References

Reviews

Massimiliano Marvasi, Pieter T Visscher, Lilliam Casillas Martinez
Exopolymeric substances (EPS) from Bacillus subtilis: polymers and genes encoding their synthesis.
FEMS Microbiol Lett: 2010, 313(1);1-9
[PubMed:20735481] [WorldCat.org] [DOI] (I p)

Original publications

The EAR RNA switch

Irnov Irnov, Wade C Winkler
A regulatory RNA required for antitermination of biofilm and capsular polysaccharide operons in Bacillales.
Mol Microbiol: 2010, 76(3);559-75
[PubMed:20374491] [WorldCat.org] [DOI] (I p)

Zasha Weinberg, Joy X Wang, Jarrod Bogue, Jingying Yang, Keith Corbino, Ryan H Moy, Ronald R Breaker
Comparative genomics reveals 104 candidate structured RNAs from bacteria, archaea, and their metagenomes.
Genome Biol: 2010, 11(3);R31
[PubMed:20230605] [WorldCat.org] [DOI] (I p)

Other original publications

Jared T Winkelman, Anna C Bree, Ashley R Bate, Patrick Eichenberger, Richard L Gourse, Daniel B Kearns
RemA is a DNA-binding protein that activates biofilm matrix gene expression in Bacillus subtilis.
Mol Microbiol: 2013, 88(5);984-97
[PubMed:23646920] [WorldCat.org] [DOI] (I p)

Munehiro Asally, Mark Kittisopikul, Pau Rué, Yingjie Du, Zhenxing Hu, Tolga Çağatay, Andra B Robinson, Hongbing Lu, Jordi Garcia-Ojalvo, Gürol M Süel
Localized cell death focuses mechanical forces during 3D patterning in a biofilm.
Proc Natl Acad Sci U S A: 2012, 109(46);18891-6
[PubMed:23012477] [WorldCat.org] [DOI] (I p)

Christine Diethmaier, Nico Pietack, Katrin Gunka, Christoph Wrede, Martin Lehnik-Habrink, Christina Herzberg, Sebastian Hübner, Jörg Stülke
A novel factor controlling bistability in Bacillus subtilis: the YmdB protein affects flagellin expression and biofilm formation.
J Bacteriol: 2011, 193(21);5997-6007
[PubMed:21856853] [WorldCat.org] [DOI] (I p)

Martin Lehnik-Habrink, Marc Schaffer, Ulrike Mäder, Christine Diethmaier, Christina Herzberg, Jörg Stülke
RNA processing in Bacillus subtilis: identification of targets of the essential RNase Y.
Mol Microbiol: 2011, 81(6);1459-73
[PubMed:21815947] [WorldCat.org] [DOI] (I p)

Onuma Chumsakul, Hiroki Takahashi, Taku Oshima, Takahiro Hishimoto, Shigehiko Kanaya, Naotake Ogasawara, Shu Ishikawa
Genome-wide binding profiles of the Bacillus subtilis transition state regulator AbrB and its homolog Abh reveals their interactive role in transcriptional regulation.
Nucleic Acids Res: 2011, 39(2);414-28
[PubMed:20817675] [WorldCat.org] [DOI] (I p)

Kazuo Kobayashi
SlrR/SlrA controls the initiation of biofilm formation in Bacillus subtilis.
Mol Microbiol: 2008, 69(6);1399-410
[PubMed:18647168] [WorldCat.org] [DOI] (I p)

Yunrong Chai, Frances Chu, Roberto Kolter, Richard Losick
Bistability and biofilm formation in Bacillus subtilis.
Mol Microbiol: 2008, 67(2);254-63
[PubMed:18047568] [WorldCat.org] [DOI] (P p)

Frances Chu, Daniel B Kearns, Steven S Branda, Roberto Kolter, Richard Losick
Targets of the master regulator of biofilm formation in Bacillus subtilis.
Mol Microbiol: 2006, 59(4);1216-28
[PubMed:16430695] [WorldCat.org] [DOI] (P p)

Daniel B Kearns, Frances Chu, Steven S Branda, Roberto Kolter, Richard Losick
A master regulator for biofilm formation by Bacillus subtilis.
Mol Microbiol: 2005, 55(3);739-49
[PubMed:15661000] [WorldCat.org] [DOI] (P p)

Dacheng Ren, Laura A Bedzyk, Peter Setlow, Stuart M Thomas, Rick W Ye, Thomas K Wood
Gene expression in Bacillus subtilis surface biofilms with and without sporulation and the importance of yveR for biofilm maintenance.
Biotechnol Bioeng: 2004, 86(3);344-64
[PubMed:15083514] [WorldCat.org] [DOI] (P p)

S S Branda, J E González-Pastor, S Ben-Yehuda, R Losick, R Kolter
Fruiting body formation by Bacillus subtilis.
Proc Natl Acad Sci U S A: 2001, 98(20);11621-6
[PubMed:11572999] [WorldCat.org] [DOI] (P p)

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