Difference between revisions of "SubtInteract"
(→Suspected hub proteins potentially involved in a large number of interactions (as deduced from a Yeast two-hybrid analysis)) |
(→Protein complexes in B. subtilis) |
||
| Line 28: | Line 28: | ||
=== DNA uptake: the [[pseudopilus]] {{PubMed|21278288,16751195}}=== | === DNA uptake: the [[pseudopilus]] {{PubMed|21278288,16751195}}=== | ||
=== [[metabolism]]: the metabolons of glycolysis and the TCA cycle {{PubMed|19193632,20933603}}=== | === [[metabolism]]: the metabolons of glycolysis and the TCA cycle {{PubMed|19193632,20933603}}=== | ||
| + | ===spore [[germination]]: the [[germinosome]]=== | ||
==Suspected hub proteins potentially involved in a large number of interactions (as deduced from a Yeast two-hybrid analysis)== | ==Suspected hub proteins potentially involved in a large number of interactions (as deduced from a Yeast two-hybrid analysis)== | ||
Revision as of 10:42, 28 June 2011
Protein-protein interactions are essential for many activities of any living cell. These interactions involve multi-protein complexes that take part in central processes such as DNA replication, transcription or translation. Protein-protein interactions may also be involved in a variety of regulatory events. Metabolic enzymes do often form transien complexes that represent a complete pathways. These complexes are called metabolon. Finally, many interactions may be of a transient nature.
Contents
- 1 Methods to detect protein-protein interactions
- 2 Visualization of protein-protein interactions in B. subtilis
- 3 Protein complexes in B. subtilis
- 3.1 DNA replication: the replisome
- 3.2 transcription: RNA polymerase
- 3.3 translation: the ribosome
- 3.4 synthesis of glutamyl-tRNA(Gln): the transamidosome (GatA-GatB-GatC)-GltX-trnS-Gln
- 3.5 RNA processing and degradation: the RNA degradosome
- 3.6 general stress response: the stressosome
- 3.7 cell division: the divisome
- 3.8 DNA uptake: the pseudopilus PubMed
- 3.9 metabolism: the metabolons of glycolysis and the TCA cycle PubMed
- 3.10 spore germination: the germinosome
- 4 Suspected hub proteins potentially involved in a large number of interactions (as deduced from a Yeast two-hybrid analysis)
- 5 Important publications
Methods to detect protein-protein interactions
- Yeast Two Hybrid System PubMed
- TAP-Tag purification PubMed
Attention: Each technique detects only about 33% of all interactions PubMed
Visualization of protein-protein interactions in B. subtilis
- interactive protein-protein interaction map
- the beta version of SubtInteract
Protein complexes in B. subtilis
DNA replication: the replisome
transcription: RNA polymerase
translation: the ribosome
synthesis of glutamyl-tRNA(Gln): the transamidosome (GatA-GatB-GatC)-GltX-trnS-Gln
RNA processing and degradation: the RNA degradosome
general stress response: the stressosome
cell division: the divisome
DNA uptake: the pseudopilus PubMed
metabolism: the metabolons of glycolysis and the TCA cycle PubMed
spore germination: the germinosome
Suspected hub proteins potentially involved in a large number of interactions (as deduced from a Yeast two-hybrid analysis)
- FruA, SwrC, XhlA, YhaP, YhgE, YkcC, YqfF, CsbC, CssS, FliZ, MreD, PpsC, RacA, Smc, YclI, YdeL, YdgH, YdbI, YesS, YkoT, YopZ, YqbD, YtdP, YueB, YwqJ, YyxA
Important publications
