EpsE

• Description: inhibitor of motility
  
  

Gene name	epsE
Synonyms	yveO
Essential	no
Product	inhibitor of motility
Function	biofilm formation
Regulation of this protein in SubtiPathways: Biofilm
MW, pI	32 kDa, 9.804
Gene length, protein length	834 bp, 278 aa
Immediate neighbours	epsF, epsD
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU34330

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • arrests flagellar rotation in a manner similar to that of a clutch, by disengaging motor force-generating elements in cells embedded in the biofilm matrix PubMed

• Protein family: glycosyltransferase 2 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: EpsE-FliG PubMed

• Localization: cell membrane, forms spots at flagellar basal bodies PubMed

Database entries

• Structure:

• UniProt: P71054

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: epsA-epsB-epsC-epsD-epsE-epsF-epsG-epsH-epsI-epsJ-epsK-epsL-epsM-epsN-epsO PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by SinR PubMed

• Regulatory mechanism:
  • SinR: transcription repression PubMed
  • AbrB: transcription repression PubMed

• Additional information:
  • induction by sequestration of SinR by SinI or SlrA PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

• Daniel Kearns, Indiana University, Bloomington, USA, homepage
• Richard Losick, Harvard Univ., Cambridge, USA homepage