Difference between revisions of "SpoIIIAG"

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Revision as of 13:53, 16 May 2013

  • Description: component of the SpoIIIA-SpoIIQ type III secretion system residing in the forespore membrane, required for SigG activation

Gene name spoIIIAG
Synonyms
Essential no
Product component of a type III secretion system
Function activation of SigG
Gene expression levels in SubtiExpress: spoIIIAG
MW, pI 25 kDa, 6.407
Gene length, protein length 687 bp, 229 aa
Immediate neighbours spoIIIAH, spoIIIAF
Sequences Protein DNA DNA_with_flanks
Genetic context
SpoIIIAG context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
SpoIIIAG expression.png
























Categories containing this gene/protein

protein secretion, sporulation proteins, membrane proteins

This gene is a member of the following regulons

SigE regulon, SpoIIID regulon

The gene

Basic information

  • Locus tag: BSU24370

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Additional information: the internal promoter is essential for sporulation, it is twice as active as the promoter in front of spoIIIAA, suggesting that SpoIIIAG and SpoIIIAH are required in larger amounts as compared to the other products of the operon PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Thierry Doan, Cecile Morlot, Jeffrey Meisner, Monica Serrano, Adriano O Henriques, Charles P Moran, David Z Rudner
Novel secretion apparatus maintains spore integrity and developmental gene expression in Bacillus subtilis.
PLoS Genet: 2009, 5(7);e1000566
[PubMed:19609349] [WorldCat.org] [DOI] (I p)

Amy H Camp, Richard Losick
A novel pathway of intercellular signalling in Bacillus subtilis involves a protein with similarity to a component of type III secretion channels.
Mol Microbiol: 2008, 69(2);402-17
[PubMed:18485064] [WorldCat.org] [DOI] (I p)

Chris Guillot, Charles P Moran
Essential internal promoter in the spoIIIA locus of Bacillus subtilis.
J Bacteriol: 2007, 189(20);7181-9
[PubMed:17693505] [WorldCat.org] [DOI] (P p)

N Illing, J Errington
The spoIIIA operon of Bacillus subtilis defines a new temporal class of mother-cell-specific sporulation genes under the control of the sigma E form of RNA polymerase.
Mol Microbiol: 1991, 5(8);1927-40
[PubMed:1766372] [WorldCat.org] [DOI] (P p)