BioI

• Description: cytochrome P450 enzyme
  
  

Gene name	bioI
Synonyms
Essential	no
Product	cytochrome P450 enzyme
Function	biosynthesis of biotin
Metabolic function and regulation of this protein in SubtiPathways: Biotin
MW, pI	44 kDa, 6.2
Gene length, protein length	1185 bp, 395 aa
Immediate neighbours	ytbQ, bioB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

biosynthesis of cofactors

This gene is a member of the following regulons

BirA regulon

The gene

Basic information

• Locus tag: BSU30190

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: formation of pimelic acid via a multiple-step oxidative cleavage of long-chain fatty acids

• Protein family: cytochrome P450 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: Fer-BioI PubMed, YkuP-BioI PubMed, AcpA-BioI PubMed

• Localization:

Database entries

• Structure: 3EJB, 3EJE (complex with AcpA) PubMed

• UniProt: P53554

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: bioW-bioA-bioF-bioD-bioB-bioI-ytbQ PubMed

• Sigma factor:

• Regulation:
  • repressed in the presence of biotin (BirA) PubMed

• Regulatory mechanism:
  • BirA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Steven Lin, John E Cronan
Closing in on complete pathways of biotin biosynthesis.
Mol Biosyst: 2011, 7(6);1811-21
[PubMed:21437340] [WorldCat.org] [DOI] (I p)