Difference between revisions of "EpsN"

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(Expression and regulation)
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=Expression and regulation=
 
=Expression and regulation=
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* '''Operon:''' ''[[epsA]]-[[epsB]]-[[epsC]]-[[epsD]]-[[epsE]]-[[epsF]]-[[epsG]]-[[epsH]]-[[epsI]]-[[epsJ]]-[[epsK]]-[[epsL]]-[[epsM]]-[[epsN]]-[[epsO]]'' {{PubMed|15661000}}
  
* '''Operon:''' ''[[epsA]]-[[epsB]]-[[epsC]]-[[epsD]]-[[epsE]]-[[epsF]]-[[epsG]]-[[epsH]]-[[epsI]]-[[epsJ]]-[[epsK]]-[[epsL]]-[[epsM]]-[[epsN]]-[[epsO]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/15661000 PubMed]
+
* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|15661000}}
 
 
* '''[[Sigma factor]]:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/15661000 PubMed]
 
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
** repressed by [[SinR]] [http://www.ncbi.nlm.nih.gov/sites/entrez/15661000 PubMed]
+
** repressed by [[SinR]] {{PubMed|15661000}}
  
* '''Regulatory mechanism:''' [[SinR]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/15661000 PubMed]
+
* '''Regulatory mechanism:'''  
** [[SinR]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/15661000 PubMed]
+
** [[SinR]]: transcription repression {{PubMed|15661000}}
 +
** [[SlrR]]: transcription activation {{PubMed|18647168}}
  
* '''Additional information:''' induction by sequestration of [[SinR]] by [[SinI]] [http://www.ncbi.nlm.nih.gov/sites/entrez/15661000 PubMed]
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* '''Additional information:'''  
 +
** induction by sequestration of [[SinR]] by [[SinI]] {{PubMed|15661000}}
 +
** activation by [[SlrR]] requires prior formation of the [[SlrR]]-[[SlrA]] complex {{PubMed|18647168}}
  
 
=Biological materials =
 
=Biological materials =

Revision as of 09:19, 30 December 2009

  • Description: extracellular polysaccharide synthesis

Gene name epsN
Synonyms yvfE
Essential no
Product unknown
Function extracellular polysaccharide synthesis
MW, pI 42 kDa, 5.843
Gene length, protein length 1164 bp, 388 aa
Immediate neighbours epsO, epsM
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YvfE context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU34230

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: degT/dnrJ/eryC1 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Richard Losick, Harvard Univ., Cambridge, USA homepage

Your additional remarks

References

Yunrong Chai, Frances Chu, Roberto Kolter, Richard Losick
Bistability and biofilm formation in Bacillus subtilis.
Mol Microbiol: 2008, 67(2);254-63
[PubMed:18047568] [WorldCat.org] [DOI] (P p)

Frances Chu, Daniel B Kearns, Steven S Branda, Roberto Kolter, Richard Losick
Targets of the master regulator of biofilm formation in Bacillus subtilis.
Mol Microbiol: 2006, 59(4);1216-28
[PubMed:16430695] [WorldCat.org] [DOI] (P p)

Daniel B Kearns, Frances Chu, Steven S Branda, Roberto Kolter, Richard Losick
A master regulator for biofilm formation by Bacillus subtilis.
Mol Microbiol: 2005, 55(3);739-49
[PubMed:15661000] [WorldCat.org] [DOI] (P p)