yxjG

yxjG
168

putative B12-independent methionine synthase

Locus
BSU_38960
Molecular weight
42.96 kDa
Isoelectric point
5.01
Protein length
378 aaSequenceBlast
Gene length
1137 bpSequenceBlast
Function
unknown
Product
putative methionine synthase
Essential
no
Synonyms
yxjG
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

List of homologs in different organisms, belongs to COG0620 (Galperin et al., 2021)

This gene is a member of the following regulons

S-box

Gene
Coordinates
3,999,350  4,000,486
The protein
Structure
1YPX (PDB) (the enzyme from Listeria monocytogenes, 49% identity)
P42318 (AlphaFold)
Modification
S-bacillithiolation upon hypochlorite stress on Cys-346 PubMed
Paralogous protein(s)
YxjH
Expression and Regulation
Operons
Genes
yxjG
Description
PubMed
Regulation
induced by methionine starvation (S-box) PubMed
the S-box RNA is degraded by RNase Y PubMed
Regulatory mechanism
S-box: RNA switch, , the S-box riboswitch binds S-adenosylmethionine resulting in termination PubMed, in S-box
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yxjG

2025-04-04 12:22:21

Jstuelk

65

c73b35012c05032f9394aded9af556aba04b6aec

DD172A67E72D799BA6CDE3355BD789009EFA1C46

Biological materials
Mutant
MGNA-B731 (yxjG::erm), available at the NBRP B. subtilis, Japan
BKE38960 (yxjG::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTGTCCATTCCTTCTT,  downstream forward: _UP4_TAAGCAAAGAAAAAAACACA
BKK38960 (yxjG::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTGTCCATTCCTTCTT,  downstream forward: _UP4_TAAGCAAAGAAAAAAACACA
References
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Time of last update: 2025-04-07 20:28:19

Author of last update: Jstuelk