yxjG
168
putative B12-independent methionine synthase
locus
BSU_38960
Molecular weight
42.96 kDa
pI
5.01
function
unknown
product
putative methionine synthase
essential
no
synonyms
yxjG
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0620 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,999,350 4,000,486
The protein
Structure
[PDB|1YPX] (the enzyme from ''Listeria monocytogenes'', 49% identity)
[AF|P42318]
Modification
S-bacillithiolation upon hypochlorite stress on Cys-346 [Pubmed|21749987]
Paralogous protein(s)
[protein|55459D8F0BBB2669EB9F25276A17F1A7EB322AF1|yxjH]
Expression and Regulation
Operons
genes
[gene|A866ACE8431EC3FF98ADC734D9322AE699ECBFAD|yxjG]
description
[Pubmed|10746760]
regulation
induced by methionine starvation ([wiki|S-box]) [Pubmed|10094622]
the [wiki|S-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]
regulatory mechanism
S-box: RNA switch, , the [wiki|S-box] [wiki|riboswitch] binds S-adenosylmethionine resulting in termination [Pubmed|10094622], in [regulon|other_regulator:S-box|S-box]
Biological materials
Mutant
MGNA-B731 (yxjG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1730 NBRP B. subtilis, Japan]
BKE38960 ([gene|A866ACE8431EC3FF98ADC734D9322AE699ECBFAD|yxjG]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE38960 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTGTCCATTCCTTCTT, downstream forward: _UP4_TAAGCAAAGAAAAAAACACA
BKK38960 ([gene|A866ACE8431EC3FF98ADC734D9322AE699ECBFAD|yxjG]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK38960 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTGTCCATTCCTTCTT, downstream forward: _UP4_TAAGCAAAGAAAAAAACACA
References
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Time of last update: 2024-11-03 02:27:38
Author of last update: Jstuelk