map

map
168

methionine aminopeptidase

Locus
BSU_01380
Molecular weight
27.26 kDa
Isoelectric point
6.39
Protein length
248 aaSequenceBlast
Gene length
747 bpSequenceBlast
Function
removal of N-terminal methionine from nascent proteins
Product
methionine aminopeptidase
Essential
no
E.C.
3.4.11.18
Synonyms
map, mapA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0024 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
146,527  147,273
Phenotypes of a mutant
essential PubMed, non-essential according to PubMed
The protein
Catalyzed reaction/ biological activity
Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides (according to UniProt)
Protein family
peptidase M24A family (with YflG, according to UniProt)
Structure
1O0X (PDB) (from Thermotoga maritima, 50% identity) PubMed
2MAT (PDB) (from Escherichia coli, 46% identity, 65% similarity) PubMed
P19994 (AlphaFold)
Paralogous protein(s)
YflG
Localization
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
Genes
rplO-secY-adk-map
Description
NULL
Regulation
RelA dependent downregulation (Class I) during stringent response PubMed
Regulatory mechanism
stringent response: negative regulation, in stringent response
Sigma factors
SigA: sigma factor, PubMed (two promoters, about 140bp and 200bp upstream of the start codon), in sigA regulon
Additional information
strongly expressed
Open in new tab

rplO→map

2025-03-09 12:09:58

ghost

117

0fcb68b7a6ea4a1daf5330728804c5d02a1c9715

678D7FB850B7A0788F1455D46D0FCE6D548E5CD2

Genes
rpsJ-rplC-rplD-rplW-rplB-rpsS-rplV-rpsC-rplP-rpmC-rpsQ-rplN-rplX-rplE-rpsN-rpsH-rplF-rplR-rpsE-rpmD-rplO-secY-adk-map-oapB-infA-rpmJ-rpsM-rpsK-S56-rpoA-rplQ
Description
PubMed
Regulation
strongly repressed in response to glucose starvation in M9 medium PubMed
likely autorepression of operon expression upon binding of excess of one ribosomal protein to the untranslated region of the mRNA PubMed
expression is reduced in motile cells as compared to non-motile cells PubMed
Regulatory mechanism
stringent response: negative regulation, in stringent response
Sigma factors
SigA: sigma factor, PubMed (two promoters, about 140bp and 200bp upstream of the start codon), in sigA regulon
Additional information
term-seq has identified a potential novel regulatory RNA element (protein-dependent leader) including an intrinsic transcription terminator upstream of rpsJ PubMed
Open in new tab

rpsJ→rplQ

2025-03-16 17:24:44

Jstuelk

145

911D53E804EEFE6335D6A0F79722F9870F387CD3

9027854E648E8BF3E4A3C8492D350C69AC71C218

Biological materials
Mutant
BKE01380 (map::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTCACGTGGGGTTTTACAGA,  downstream forward: _UP4_TAGATGATGAAAATAATTCC
BKK01380 (map::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTCACGTGGGGTTTTACAGA,  downstream forward: _UP4_TAGATGATGAAAATAATTCC
References
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2F2455E189FC61A6B1EC6B66412FC662E44BD3F6

Page visits: 5777

Time of last update: 2025-03-17 22:30:35

Author of last update: Jstuelk