rplO

rplO
168

ribosomal protein uL15

Locus
BSU_01350
Molecular weight
15.24 kDa
Isoelectric point
10.79
Protein length
146 aaSequenceBlast
Gene length
441 bpSequenceBlast
Function
translation
Product
ribosomal protein L15 (uL15)
Essential
no
Synonyms
rplO
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0200 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
144,085 → 144,525
The protein
Protein family
universal ribosomal protein uL15 family (single member, according to UniProt)
Structure
3J9W (PDB) (the ribosome) PubMed
P19946 (AlphaFold)
Expression and Regulation
Operons
Genes
rplO-secY-adk-map
Description
NULL
Regulation
RelA dependent downregulation (Class I) during stringent response PubMed
Regulatory mechanism
stringent response: negative regulation, in stringent response
Sigma factors
SigA: sigma factor, PubMed (two promoters, about 140bp and 200bp upstream of the start codon), in sigA regulon
Additional information
strongly expressed
Open in new tab

rplO→map

2025-03-09 12:09:58

ghost

117

0fcb68b7a6ea4a1daf5330728804c5d02a1c9715

678D7FB850B7A0788F1455D46D0FCE6D548E5CD2

Genes
rpsJ-rplC-rplD-rplW-rplB-rpsS-rplV-rpsC-rplP-rpmC-rpsQ-rplN-rplX-rplE-rpsN-rpsH-rplF-rplR-rpsE-rpmD-rplO-secY-adk-map-oapB-infA-rpmJ-rpsM-rpsK-S56-rpoA-rplQ
Description
PubMed
Regulation
strongly repressed in response to glucose starvation in M9 medium PubMed
likely autorepression of operon expression upon binding of excess of one ribosomal protein to the untranslated region of the mRNA PubMed
expression is reduced in motile cells as compared to non-motile cells PubMed
Regulatory mechanism
stringent response: negative regulation, in stringent response
Sigma factors
SigA: sigma factor, PubMed (two promoters, about 140bp and 200bp upstream of the start codon), in sigA regulon
Additional information
term-seq has identified a potential novel regulatory RNA element (protein-dependent leader) including an intrinsic transcription terminator upstream of rpsJ PubMed
Open in new tab

rpsJ→rplQ

2025-04-02 13:05:40

Jstuelk

147

911D53E804EEFE6335D6A0F79722F9870F387CD3

9027854E648E8BF3E4A3C8492D350C69AC71C218

Biological materials
Mutant
BKE01350 (ΔrplO::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTAGGACACCTCCTTATC,  downstream forward: _UP4_GGCGGTACAGCTGAGGTGAT
BKK01350 (ΔrplO::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTAGGACACCTCCTTATC,  downstream forward: _UP4_GGCGGTACAGCTGAGGTGAT
References
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Page visits: 6319

Time of last update: 2025-04-04 10:52:08

Author of last update: Jstuelk