rqcH

rqcH
168

ribosome-associated protein quality control factor, selects tRNAAla during RQC elongation

Locus
BSU_15640
Molecular weight
65.30 kDa
Isoelectric point
8.98
Protein length
572 aaSequenceBlast
Gene length
1719 bpSequenceBlast
Function
rescue of stalled ribosomes
Product
ribosome-associated protein quality control factor
Essential
no
Synonyms
rqcH, yloA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1293 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,636,131  1,637,849
Phenotypes of a mutant
defective in biofilm formation PubMed
a rcqH smpB double mutant has a severe growth defect PubMed
The protein
Catalyzed reaction/ biological activity
senses stalled translation intermediates and recruits tRNAAla(UGC) to modify nascent-chain C termini with a polyalanine degron for degradation PubMed
Protein family
NEMF family (single member, according to UniProt)
Structure
8S1U (PDB) YlmH and RqcH bound to the 50S ribosome subunit bioRxiv
O34693 (AlphaFold)
Expression and Regulation
Operons
Genes
rqcH
Description
PubMed
Regulation
AbrB: repression during logarithmic growth PubMed
DegU: repression during stationary phase PubMed
Regulatory mechanism
AbrB: repression, PubMed, in abrB regulon
DegU: repression, PubMed, in degU regulon
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rqcH

2025-03-15 13:22:52

Jstuelk

88

29a04bf16d345a1e9def0254967c2d97c5ec4025

16AF2F8748CFED90DEDBEEF2375C91492E353A84

Biological materials
Mutant
MGNA-B371 (yloA::erm), available at the NBRP B. subtilis, Japan
BKE15640 (rqcH::erm trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATACACCCTCTCATTCTT,  downstream forward: _UP4_TGAGCCGCATAAAGAAAAGC
BKK15640 (rqcH::kan trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATACACCCTCTCATTCTT,  downstream forward: _UP4_TGAGCCGCATAAAGAAAAGC
References
Reviews
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Original Publications
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003047E90159765F9F0E499FD760F95FFF4AC360

Page visits: 3956

Time of last update: 2025-03-16 12:34:07

Author of last update: Jstuelk