smpB

smpB
168

tmRNA-binding protein

locus
BSU_33600
Molecular weight
17.95 kDa
pI
10.42
Protein length
Gene length
function
trans-translation
product
tmRNA-binding protein
essential
no
synonyms
smpB, yvaI

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0691 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,451,248 → 3,451,718
Phenotypes of a mutant
impaired growth at high and low temperatures [Pubmed|15805528]
inactivation of ''[gene|D19519B5A776D6E8EC2BFE7A7D67469CA0A6459A|smpB]'' reduces sporulation efficiency to 23% that of wild type cells; delayed entry into sporulation [Pubmed|26735940]
a [gene|F7F780D11A6B11EB62CFA5202D1243A381AB40E1|brfA] [gene|D19519B5A776D6E8EC2BFE7A7D67469CA0A6459A|smpB] double mutant has a severe growth defect [pubmed|31776341]
a [gene|search|rcqH] [gene|D19519B5A776D6E8EC2BFE7A7D67469CA0A6459A|smpB] double mutant has a severe growth defect [pubmed|39026760]
The protein
Protein family
smpB family (single member, according to UniProt)
Structure
[PDB|1P6V] (from ''Aquifex aeolicus'', 55% identity, complex with the tRNA domain of [protein|5C12BB19B975F7FEBCFA119DAC66338C20FCECFA|ssrA]) [Pubmed|12904796]
[AF|O32230]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|D8851F453E5EF18D36F25834A8CB9C61EAF15E44|secG]-[gene|C0B6520E74CE9F7EE3DEC8C0D83E0770F241C4E8|yvaK]-[gene|0472A4346ADA1DC41C9D00E8F3222A0EBB76DFA8|rnr]-[gene|D19519B5A776D6E8EC2BFE7A7D67469CA0A6459A|smpB]-[gene|5C12BB19B975F7FEBCFA119DAC66338C20FCECFA|ssrA]
description
[Pubmed|17369301]
regulation
''[protein|search|yvaK]'': induced by stress ([protein|search|SigB]) [Pubmed|11544224]
sigma factors
[protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, [Pubmed|11544224], in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]
Open in new tab

[gene|D8851F453E5EF18D36F25834A8CB9C61EAF15E44|secG]→[gene|5C12BB19B975F7FEBCFA119DAC66338C20FCECFA|ssrA]

2024-10-05 05:35:57

ghost

82

5b644e318b4e03f8229d1f7804ae24c07fdeb5dd

238E5C487975E311E1EBD32D07A14F173B276AD8

genes
[gene|D19519B5A776D6E8EC2BFE7A7D67469CA0A6459A|smpB]-[gene|5C12BB19B975F7FEBCFA119DAC66338C20FCECFA|ssrA]
description
[Pubmed|17369301]
regulation
''[protein|search|yvaK]'': [protein|search|SigB] [Pubmed|11544224]
Open in new tab

[gene|D19519B5A776D6E8EC2BFE7A7D67469CA0A6459A|smpB]→[gene|5C12BB19B975F7FEBCFA119DAC66338C20FCECFA|ssrA]

2024-10-05 05:35:43

ghost

101

79314a3579b82df76918096ecacc3b7bd94ed6b9

3806406E5CBA52123D5A75B46E96105A42099755

genes
[gene|C0B6520E74CE9F7EE3DEC8C0D83E0770F241C4E8|yvaK]-[gene|0472A4346ADA1DC41C9D00E8F3222A0EBB76DFA8|rnr]-[gene|D19519B5A776D6E8EC2BFE7A7D67469CA0A6459A|smpB]-[gene|5C12BB19B975F7FEBCFA119DAC66338C20FCECFA|ssrA]
description
[Pubmed|17369301]
regulation
''[protein|search|yvaK]'': induced by stress ([protein|search|SigB]) [Pubmed|11544224]
Open in new tab

[gene|C0B6520E74CE9F7EE3DEC8C0D83E0770F241C4E8|yvaK]→[gene|5C12BB19B975F7FEBCFA119DAC66338C20FCECFA|ssrA]

2024-10-05 05:35:41

ghost

83

d8154c99b5174d2c9614c36d072e1652057bf9f1

E221678AD029D36CCA9613ABC361FB40A01462F7

Biological materials
Mutant
MGNA-A494 (yvaI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/494 NBRP B. subtilis, Japan]
BKE33600 (Δ[gene|D19519B5A776D6E8EC2BFE7A7D67469CA0A6459A|smpB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE33600 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGCCAGAACCTCCTCTCT,  downstream forward: _UP4_TAAAGGCATAGTGCTTGATT
BKK33600 (Δ[gene|D19519B5A776D6E8EC2BFE7A7D67469CA0A6459A|smpB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK33600 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGCCAGAACCTCCTCTCT,  downstream forward: _UP4_TAAAGGCATAGTGCTTGATT
labs
[wiki|Chet Price], Davis, USA [http://foodscience.ucdavis.edu/price_lab/pricelab.html homepage]
References
Reviews
12730326,10881189,33815344,34488571
Original Publications
18977770,11395451,17369301,10881189,15805528,12904796,26735940

D19519B5A776D6E8EC2BFE7A7D67469CA0A6459A

Page visits: 2766

Time of last update: 2024-10-07 16:29:12

Author of last update: Jstuelk