yvdF
168
glucan 1,4-alpha-maltohydrolase, neopullulanase, maltogenic amylase
locus
BSU_34620
Molecular weight
68.68 kDa
pI
5.18
function
starch and maltodextrin utilization
product
glucan 1,4-alpha-maltohydrolase, neopullulanase, maltogenic amylase
essential
no
synonyms
yvdF, bbmA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0366 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,555,902 3,557,671
The protein
Protein family
[wiki|glycosyl hydrolase 13 family] (according to UniProt)
Structure
[PDB|1J0J] (from G. stearothermophilus, 56% identity, complex with maltotetraose), [PDB|1J0H] (from G. stearothermophilus, 56% identity)
[AF|O06988]
[wiki|Localization]
on both sides of the cytoplasmic membrane and in the periplasm during vegetative growth but in the cytoplasm of prespores [Pubmed|19465663]
Expression and Regulation
Operons
genes
[gene|EA1BEC9FCFD2A32142A8A8E6D7362DB37E02052A|mdxR]-[gene|A577441DE4ABAF624D507DB80FBED2A652BCF385|yvdF]-[gene|5771E1976D986638B13ADDE97EDE32709E355923|mdxE]-[gene|EA2DD9892C1128ADCD6C8BC61248EBE45339E6D6|mdxF]-[gene|D72CFFE8ED3EC0280AE92C05A136D66ED1F62251|mdxG]-[gene|68C6FD5D4D9491EE15A5015447959A45C36EA702|yvdJ]-[gene|5F615BB886EC4F7000AAD42258DB8E47616552DE|yvdK]-[gene|6E5AB4620F9A896C32CD77AF314C67035814AE0A|malL]-[gene|CAFAE305B1771DD89E81F75A6713161393BE99A0|pgcM]
description
[Pubmed|16707683]
regulation
induced in the presence of starch, β-cyclomaltodextrin, or maltose [Pubmed|36723711,9573215]
regulatory mechanism
[protein|EA1BEC9FCFD2A32142A8A8E6D7362DB37E02052A|mdxR]: activation, [pubmed|36723711], in [regulon|protein:EA1BEC9FCFD2A32142A8A8E6D7362DB37E02052A|mdxR regulon]
Biological materials
Mutant
MGNA-B628 (yvdF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1627 NBRP B. subtilis, Japan]
BKE34620 ([gene|A577441DE4ABAF624D507DB80FBED2A652BCF385|yvdF]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE34620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTCCCCCTTTGATTT, downstream forward: _UP4_TAACATTCTGTTATCGGTAA
BKK34620 ([gene|A577441DE4ABAF624D507DB80FBED2A652BCF385|yvdF]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK34620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTCCCCCTTTGATTT, downstream forward: _UP4_TAACATTCTGTTATCGGTAA
References
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Time of last update: 2024-10-07 06:50:20
Author of last update: Robert.warneke