purD

purD
168

phosphoribosylglycinamide synthetase

Locus
BSU_06530
Molecular weight
45.12 kDa
Isoelectric point
4.6
Protein length
422 aaSequenceBlast
Gene length
1269 bpSequenceBlast
Function
purine biosynthesis
Product
phosphoribosylglycinamide synthetase
Essential
no
E.C.
6.3.4.13
Synonyms
purD
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0151 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
710,148 711,416
The protein
Catalyzed reaction/ biological activity
5-phospho-D-ribosylamine + ATP + glycine --> ADP + H+ + N1-(5-phospho-D-ribosyl)glycinamide + phosphate (according to UniProt)
Protein family
GARS family (single member, according to UniProt)
Domains
ATP-grasp doamin (aa 107-313) (according to UniProt)
Structure
2XCL (PDB)
P12039 (AlphaFold)
Additional information
belongs to the 100 most abundant proteins PubMed
Expression and Regulation
Operons
Genes
purE-purK-purB-purC-purS-purQ-purL-purF-purM-purN-purH-purD
Description
PubMed
Regulation
expression activated by glucose (4.4 fold) PubMed
Regulatory mechanism
G-box: RNA switch, (riboswitch) PubMed, in G-box
PurR: repression, (molecular inducer: PRPP) PubMed, in purR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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purE→purD

2025-04-17 09:05:23

Jstuelk

232

ace69dc80f4f087486c218727fa370a7a9630d21

EDD8EC37B0E5B38941D5827C265AB2F23CF23EA7

Biological materials
Mutant
BKE06530 (purD::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TACATTCACGTCGTTTTCAT, downstream forward: _UP4_TAAGTGAGGAAAACCCGCAG
BKK06530 (purD::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TACATTCACGTCGTTTTCAT, downstream forward: _UP4_TAAGTGAGGAAAACCCGCAG
References
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9BBA22B09C6DD6C36E043D44B0F54BD0FFA5BA0E

Page visits: 6277

Time of last update: 2025-04-16 01:08:21

Author of last update: Melvin.boenninger