fadN

fadN
168

3-hydroxyacyl-CoA dehydrogenase (acetoacetyl-CoA)

Locus
BSU_32840
Molecular weight
89.96 kDa
Isoelectric point
6.53
Protein length
815 aaSequenceBlast
Gene length
2448 bpSequenceBlast
Function
fatty acid degradation
Product
3-hydroxyacyl-CoA dehydrogenase (acetoacetyl-CoA)
Essential
no
E.C.
1.1.1.35
Synonyms
fadN, yusL
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

List of homologs in different organisms, belongs to COG1250 (Galperin et al., 2021)

This gene is a member of the following regulons

SigA regulon, CcpA regulon, SdpR regulon, FadR regulon

Gene
Coordinates
3,370,025  3,372,472
The protein
Catalyzed reaction/ biological activity
(3S)-hydroxyacyl-CoA + NAD+ --> 3-oxoacyl-CoA + H+ + NADH (according to UniProt)
Protein family
3-hydroxyacyl-CoA dehydrogenase family (with MmgB, according to UniProt)
Cofactors
FMN or FAD PubMed
Structure
O32178 (AlphaFold)
Expression and Regulation
Operons
Genes
fadN-fadA-fadE
Description
PubMed
Regulation
''fadN'': induced by long-chain fatty acids (FadR) PubMed
Regulatory mechanism
SdpR: activation, PubMed, in sdpR regulon
FadR: repression, PubMed, in fadR regulon
CcpA: repression, PubMed, in ccpA regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Open in new tab

fadN→fadE

2025-04-03 19:54:19

ghost

102

5f1e349b432a9058e9ca7ab2008a19fa9609b3d3

64A593E7BDE4F4B739AFAF65983E90DDA2D2CE72

Genes
fadM-fadN-fadA-fadE
Description
PubMed
Regulation
''fadM'': induced by long-chain fatty acids (FadR) PubMed
Regulatory mechanism
FadR: repression, PubMed, in fadR regulon
SdpR: activation, PubMed, in sdpR regulon
Open in new tab

fadM→fadE

2025-03-16 11:32:37

ghost

101

9bfdfb93c4077983c0e3142e433ac74ae81a5d62

8E61A92B7F4E3CA1E4373E8AFCCD07598481ACA4

Biological materials
Mutant
MGNA-A604 (yusL::erm), available at the NBRP B. subtilis, Japan
BKE32840 (fadN::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGAGATATCCCCCTTGAA,  downstream forward: _UP4_CCTTTACGGAATTAGGAGGG
BKK32840 (fadN::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGAGATATCCCCCTTGAA,  downstream forward: _UP4_CCTTTACGGAATTAGGAGGG
References
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4787161A022E938A6D58A505D92369F889C7DE04

Page visits: 5780

Time of last update: 2025-04-07 14:37:01

Author of last update: Melvin.boenninger