fadN
168
3-hydroxyacyl-CoA dehydrogenase (acetoacetyl-CoA)
locus
BSU_32840
Molecular weight
89.96 kDa
pI
6.53
function
fatty acid degradation
product
3-hydroxyacyl-CoA dehydrogenase (acetoacetyl-CoA)
essential
no
ec
1.1.1.35
synonyms
fadN, yusL
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1250 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,370,025 3,372,472
The protein
Catalyzed reaction/ biological activity
(3S)-hydroxyacyl-CoA + NAD+ --> 3-oxoacyl-CoA + H+ + NADH (according to UniProt)
Protein family
3-hydroxyacyl-CoA dehydrogenase family (with [protein|FB95ABDC8557F26525E8B211595C130A7203ABC0|mmgB], according to UniProt)
[wiki|Cofactors]
FMN or FAD [Pubmed|21635694]
Structure
[AF|O32178]
Expression and Regulation
Operons
genes
[gene|4787161A022E938A6D58A505D92369F889C7DE04|fadN]-[gene|07498A0A3CB598A6E388540124A4F56E781C86FA|fadA]-[gene|B5325CBF1408E2CA227EB462092E209F4C87E797|fadE]
description
[Pubmed|17189250]
regulation
''[protein|search|fadN]'': induced by long-chain fatty acids ([protein|search|FadR]) [Pubmed|17189250]
regulatory mechanism
[protein|29A3EC8FB0C763A973273AA14EAEB904847C3002|sdpR]: activation, [Pubmed|12817086], in [regulon|protein:29A3EC8FB0C763A973273AA14EAEB904847C3002|sdpR regulon]
[protein|8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|fadR]: repression, [Pubmed|17189250], in [regulon|protein:8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|fadR regulon]
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|21398533], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|17189250], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
genes
[gene|F8C51A86268DC6B2152B139D2D0B25CC3D69A5D5|fadM]-[gene|4787161A022E938A6D58A505D92369F889C7DE04|fadN]-[gene|07498A0A3CB598A6E388540124A4F56E781C86FA|fadA]-[gene|B5325CBF1408E2CA227EB462092E209F4C87E797|fadE]
description
[Pubmed|17189250]
regulation
''[protein|search|fadM]'': induced by long-chain fatty acids ([protein|search|FadR]) [Pubmed|17189250]
regulatory mechanism
[protein|8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|fadR]: repression, [Pubmed|17189250], in [regulon|protein:8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|fadR regulon]
[protein|29A3EC8FB0C763A973273AA14EAEB904847C3002|sdpR]: activation, [Pubmed|12817086], in [regulon|protein:29A3EC8FB0C763A973273AA14EAEB904847C3002|sdpR regulon]
Biological materials
Mutant
MGNA-A604 (yusL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/604 NBRP B. subtilis, Japan]
BKE32840 ([gene|4787161A022E938A6D58A505D92369F889C7DE04|fadN]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE32840 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAGATATCCCCCTTGAA, downstream forward: _UP4_CCTTTACGGAATTAGGAGGG
BKK32840 ([gene|4787161A022E938A6D58A505D92369F889C7DE04|fadN]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK32840 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAGATATCCCCCTTGAA, downstream forward: _UP4_CCTTTACGGAATTAGGAGGG
References
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Time of last update: 2024-12-20 07:27:06
Author of last update: Melvin.boenninger