fadM
168
fatty acid degradation, similar to proline dehydrogenase
locus
BSU_32850
Molecular weight
34.30 kDa
pI
9.38
function
fatty acid degradation
product
unknown
essential
no
synonyms
fadM, yusM
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0506 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,372,740 → 3,373,648
The protein
Catalyzed reaction/ biological activity
quinone + L-proline --> (S)-1-pyrroline-5-carboxylate + quinol + H+ (according to UniProt)
Protein family
proline dehydrogenase family (with [protein|1F548515402950572E7212901729B2480432D1B9|putB], according to UniProt)
[wiki|Cofactors]
FAD (according to UniProt)
Structure
[PDB|4H6Q] (from Deinococcus radiodurans, 48% identity) [pubmed|23151026]
[AF|O32179]
Paralogous protein(s)
[protein|1F548515402950572E7212901729B2480432D1B9|putB]
Expression and Regulation
Operons
genes
[gene|F8C51A86268DC6B2152B139D2D0B25CC3D69A5D5|fadM]-[gene|4787161A022E938A6D58A505D92369F889C7DE04|fadN]-[gene|07498A0A3CB598A6E388540124A4F56E781C86FA|fadA]-[gene|B5325CBF1408E2CA227EB462092E209F4C87E797|fadE]
description
[Pubmed|17189250]
regulation
''[protein|search|fadM]'': induced by long-chain fatty acids ([protein|search|FadR]) [Pubmed|17189250]
regulatory mechanism
[protein|8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|fadR]: repression, [Pubmed|17189250], in [regulon|protein:8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|fadR regulon]
[protein|29A3EC8FB0C763A973273AA14EAEB904847C3002|sdpR]: activation, [Pubmed|12817086], in [regulon|protein:29A3EC8FB0C763A973273AA14EAEB904847C3002|sdpR regulon]
Biological materials
Mutant
MGNA-A598 (yusM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/598 NBRP B. subtilis, Japan]
BKE32850 (Δ[gene|F8C51A86268DC6B2152B139D2D0B25CC3D69A5D5|fadM]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE32850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCGACTCCCTCCCGCCG, downstream forward: _UP4_TGAAAGCAGCCGTGGGAAGC
BKK32850 (Δ[gene|F8C51A86268DC6B2152B139D2D0B25CC3D69A5D5|fadM]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK32850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCGACTCCCTCCCGCCG, downstream forward: _UP4_TGAAAGCAGCCGTGGGAAGC
References
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Time of last update: 2024-12-30 18:58:13
Author of last update: Melvin.boenninger