lgt
168
prolipoprotein diacylglyceryl transferase
locus
BSU_34990
Molecular weight
30.47 kDa
pI
8.96
function
lipomodification of lipoproteins
product
prolipoprotein diacylglyceryl transferase
essential
no
ec
2.4.99.-
synonyms
lgt, gerF, yvoC
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0682 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,593,419 3,594,228
Phenotypes of a mutant
reduced ability of spores to germinate with alanine due ti reduced activity of the [protein|EEDE6A22E91992ABCA677CAB7917B7168C5CB868|gerAA]-[protein|5FF739B8C087207039BB6DC293EE77F59337ED0B|gerAB]-[protein|D937E26DF69D49B1341B37596AC6A8FBEEF8BA2E|gerAC] germinant receptor [Pubmed|15126458]
inactivation of ''[gene|2EB790AD1872ABCB57B784296C04C773AEA9C22B|lgt]'' reduces sporulation efficiency to 7.7% that of wild type cells [Pubmed|26735940]
The protein
Catalyzed reaction/ biological activity
1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycerol) + L-cysteinyl-[prolipoprotein] --> H+ + S-1,2-diacyl-sn-glyceryl-L-cysteinyl-[prolipoprotein] + sn-glycerol 1-phosphate (according to UniProt)
Protein family
lgt family (single member, according to UniProt)
Structure
[PDB|5AZB] (from E. coli, 29% identity) [pubmed|26729647]
[AF|O34752]
[wiki|Localization]
membrane associated [Pubmed|18763711]
Expression and Regulation
Operons
genes
[gene|771F205F818A755A661B8E1C95365A4F6AEB05C7|hprK]-[gene|2EB790AD1872ABCB57B784296C04C773AEA9C22B|lgt]-[gene|FA32AD908196BDA5C2C7C26EA08FB8416182CC3D|yvoD]-[gene|F3970DC380899E0505E80EB327AE27AFDE0F166F|yvoE]-[gene|C6B0EF2CD33D0F5455726167AE8F510619C3F6CA|yvoF]
description
[Pubmed|9465101]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|23667565], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
MGNA-A388 (lgt::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/388 NBRP B. subtilis, Japan]
GP851 (Δ[gene|2EB790AD1872ABCB57B784296C04C773AEA9C22B|lgt]-[gene|FA32AD908196BDA5C2C7C26EA08FB8416182CC3D|yvoD]-[gene|F3970DC380899E0505E80EB327AE27AFDE0F166F|yvoE]-[gene|C6B0EF2CD33D0F5455726167AE8F510619C3F6CA|yvoF])::aphA3), available in [wiki|Jörg Stülke]'s lab
1G22 ( ''lgt''::''erm''), [Pubmed|15126458], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1G22&Search=1G22 BGSC]
BKE34990 (Δ[gene|2EB790AD1872ABCB57B784296C04C773AEA9C22B|lgt]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE34990 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CGCTTCATTCATTTAACGCC, downstream forward: _UP4_TAGACGTCAACAAAGGGGGA
BKK34990 (Δ[gene|2EB790AD1872ABCB57B784296C04C773AEA9C22B|lgt]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK34990 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CGCTTCATTCATTTAACGCC, downstream forward: _UP4_TAGACGTCAACAAAGGGGGA
References
Reviews
Original Publications
Lgt in other organisms
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Time of last update: 2024-12-21 22:21:51
Author of last update: Melvin.boenninger