hprK
168
HPr kinase/ phosphorylase
locus
BSU_35000
Molecular weight
34.55 kDa
pI
4.91
function
carbon catabolite repression,phosphorylation of [protein|29B793660E4D30C0656248F3EF403FEF76FB9025|HPr] and [protein|A269774F2FDC94F93BA5F1360FFFE754B50383AD|crh] proteins at Ser46
product
[protein|29B793660E4D30C0656248F3EF403FEF76FB9025|HPr] kinase/ phosphorylase
essential
no
synonyms
hprK, ptsK, yvoB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1493 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,594,242 3,595,174
Phenotypes of a mutant
no carbon catabolite repression [Pubmed|9570401]
delay in spore [wiki|germination] [Pubmed|26381121]
The protein
Catalyzed reaction/ biological activity
[HPr protein]-L-serine + ATP --> [HPr protein]-O-phospho-L-serine + ADP + H+ (according to UniProt)
[HPr protein]-O-phospho-L-serine + H+ + phosphate --> [HPr protein]-L-serine + diphosphate (according to UniProt)
Protein family
HPrK/P family (single member, according to UniProt)
Structure
[PDB|1KNX] (from Mycoplasma pneumoniae) [pubmed|12589763]
[PDB|1KKM] (complex of Lactobacillus casei HprK with B. subtilis [protein|29B793660E4D30C0656248F3EF403FEF76FB9025|HPr]-Ser-P)
[PDB|1KKL] (complex of Lactobacillus casei HprK with B. subtilis [protein|29B793660E4D30C0656248F3EF403FEF76FB9025|HPr])
[AF|O34483]
Expression and Regulation
Operons
genes
[gene|771F205F818A755A661B8E1C95365A4F6AEB05C7|hprK]-[gene|2EB790AD1872ABCB57B784296C04C773AEA9C22B|lgt]-[gene|FA32AD908196BDA5C2C7C26EA08FB8416182CC3D|yvoD]-[gene|F3970DC380899E0505E80EB327AE27AFDE0F166F|yvoE]-[gene|C6B0EF2CD33D0F5455726167AE8F510619C3F6CA|yvoF]
description
[Pubmed|9465101]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|23667565], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
MGNA-A328 (hprK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/328 NBRP B. subtilis, Japan]
GP202 (Δ[gene|771F205F818A755A661B8E1C95365A4F6AEB05C7|hprK]::spc) [Pubmed|12055300], available in [wiki|Jörg Stülke]'s lab
GP858 (Δ[gene|771F205F818A755A661B8E1C95365A4F6AEB05C7|hprK]::aphA3) [Pubmed|18757537], available in [wiki|Jörg Stülke]'s lab
GP82 (Δ[gene|771F205F818A755A661B8E1C95365A4F6AEB05C7|hprK]::cat) [Pubmed|21992469], available in [wiki|Jörg Stülke]'s lab
BKE35000 (Δ[gene|771F205F818A755A661B8E1C95365A4F6AEB05C7|hprK]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE35000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGATATGTTCCTCCTGTT, downstream forward: _UP4_CAAGAAGAATAGGAGTTGGC
BKK35000 (Δ[gene|771F205F818A755A661B8E1C95365A4F6AEB05C7|hprK]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK35000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGATATGTTCCTCCTGTT, downstream forward: _UP4_CAAGAAGAATAGGAGTTGGC
Expression vectors
for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [wiki|SPINE], in [wiki|pGP380]: pGP642, available in [wiki|Jörg Stülke]'s lab
for expression/ purification of mutant HprK-G158A from ''B. subtilis'' with N-terminal Strep-tag, for [wiki|SPINE], in [wiki|pGP380]: pGP650, available in [wiki|Jörg Stülke]'s lab
for expression/ purification from ''E. coli'' with N-terminal His-tag, in [wiki|pWH844]: pGP205, available in [wiki|Jörg Stülke]'s lab [pubmed|12055300]
for expression, purification of the N-terminal domain in ''E. coli'' with N-terminal His-tag, in [wiki|pWH844]: pGP218, available in [wiki|Jörg Stülke]'s lab
lacZ fusion
pGP201 (in [wiki|pAC5], [Pubmed|12055300]) available in [wiki|Jörg Stülke]'s lab
pGP202 (in [wiki|pAC6], [Pubmed|12055300]) available in [wiki|Jörg Stülke]'s lab
Antibody
available in [wiki|Jörg Stülke]'s lab [Pubmed|12055300]
labs
[wiki|Jörg Stülke], University of Göttingen, Germany [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]
[wiki|Anne Galinier], University of Marseille, France
References
Reviews
Structural Analysis of HPrK
General Analysis, Physiology
Enzymatic Properties, Mutation Analysis
HprK as a Target For Antimicrobial Compounds
Page visits: 5278
Time of last update: 2024-10-07 14:24:39
Author of last update: Jstuelk