SubtInteract

Protein-protein interactions are essential for many activities of any living cell. These interactions involve multi-protein complexes that take part in central processes such as DNA replication, transcription or translation. Protein-protein interactions may also be involved in a variety of regulatory events. Metabolic enzymes do often form transien complexes that represent a complete pathways. These complexes are called metabolon. Finally, many interactions may be of a transient nature.

Now online: A description of SubtiWiki, SubtiPathways, and SubtInteract in the 2012 Database issue of Nucleic Acids Research <pubmed> 22096228 </pubmed>

Methods to detect protein-protein interactions

• Bacterial Two Hybrid System: BACTH PubMed

• Yeast Two Hybrid System PubMed

• SPINE: A method to detect in vivo protein-protein interactions after cross-linking PubMed

• TAP-Tag purification PubMed

• in vivo detection of protein-protein interaction using DivIVA and GFP PubMed

Attention: Each technique detects only about 33% of all interactions PubMed

Visualization of protein-protein interactions in B. subtilis

• interactive protein-protein interaction map
• the beta version of SubtInteract

Suspected hub proteins potentially involved in a large number of interactions (as deduced from a Yeast two-hybrid analysis)

• FruA, SwrC, XhlA, YhaP, YhgE, YkcC, YqfF, CsbC, CssS, FliZ, MreD, PpsC, RacA, Smc, YclI, YdeL, YdgH, YdbI, YesS, YkoT, YopZ, YqbD, YtdP, YueB, YwqJ, YyxA

<pubmed> 21630458</pubmed>

Important publications

<pubmed>20658969 18228443 18219467 17953394 15264234 14706816 12127457 11827824 11377797 11306254 10851163 10842303 18366733 10694888 2200717 2441660 20969605 15451506 9787636 22790590 23808343 </pubmed>