SubtInteract: Difference between revisions
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=== [[DNA replication]]: the [[replisome]]=== | === [[DNA replication]]: the [[replisome]]=== | ||
=== [[transcription]]: [[RNA polymerase]]=== | === [[transcription]]: [[RNA polymerase]]=== | ||
===[[translation]]: the [[ribosomal proteins|ribosome]]=== | === [[translation]]: the [[ribosomal proteins|ribosome]]=== | ||
=== synthesis of glutamyl-tRNA(Gln): the [[transamidosome]] ([[GatA]]-[[GatB]]-[[GatC]])-[[GltX]]-[[trnS-Gln]]=== | |||
=== [[RNases|RNA processing and degradation]]: the [[RNA degradosome]]=== | === [[RNases|RNA processing and degradation]]: the [[RNA degradosome]]=== | ||
=== [[General stress proteins (controlled by SigB)|general stress response]]: the [[stressosome]]=== | === [[General stress proteins (controlled by SigB)|general stress response]]: the [[stressosome]]=== | ||
=== [[cell division]]: the [[divisome]]=== | === [[cell division]]: the [[divisome]]=== | ||
===DNA uptake: the [[pseudopilus]] {{PubMed|21278288,16751195}}=== | === DNA uptake: the [[pseudopilus]] {{PubMed|21278288,16751195}}=== | ||
=== [[metabolism]]: the metabolons of glycolysis and the TCA cycle {{PubMed|19193632,20933603}}=== | === [[metabolism]]: the metabolons of glycolysis and the TCA cycle {{PubMed|19193632,20933603}}=== | ||
==Important publications== | ==Important publications== | ||
<pubmed>20658969 18228443 18219467 17953394 15264234 14706816 12127457 11827824 11377797 11306254 10851163 10842303 18366733 10694888 2200717 2441660 20969605 15451506 </pubmed> | <pubmed>20658969 18228443 18219467 17953394 15264234 14706816 12127457 11827824 11377797 11306254 10851163 10842303 18366733 10694888 2200717 2441660 20969605 15451506 </pubmed> | ||
Revision as of 10:03, 24 April 2011
Protein-protein interactions are essential for many activities of any living cell. These interactions involve multi-protein complexes that take part in central processes such as DNA replication, transcription or translation. Protein-protein interactions may also be involved in a variety of regulatory events. Metabolic enzymes do often form transien complexes that represent a complete pathways. These complexes are called metabolon. Finally, many interactions may be of a transient nature.
Methods to detect protein-protein interactions
- Yeast Two Hybrid System PubMed
- TAP-Tag purification PubMed
Attention: Each technique detects only about 33% of all interactions PubMed
Visualization of protein-protein interactions in B. subtilis
- the beta version of an interactive protein-protein interaction map (just replace "PtsH" in the browser line by the protein of your interest)
- the beta version of SubtInteract
Protein complexes in B. subtilis
DNA replication: the replisome
transcription: RNA polymerase
translation: the ribosome
synthesis of glutamyl-tRNA(Gln): the transamidosome (GatA-GatB-GatC)-GltX-trnS-Gln
RNA processing and degradation: the RNA degradosome
general stress response: the stressosome
cell division: the divisome
DNA uptake: the pseudopilus PubMed
metabolism: the metabolons of glycolysis and the TCA cycle PubMed
Important publications
<pubmed>20658969 18228443 18219467 17953394 15264234 14706816 12127457 11827824 11377797 11306254 10851163 10842303 18366733 10694888 2200717 2441660 20969605 15451506 </pubmed>