SubtInteract: Difference between revisions
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'''Attention:''' Each technique detects only about 33% of all interactions {{PubMed|20805792,19060903}} | '''Attention:''' Each technique detects only about 33% of all interactions {{PubMed|20805792,19060903}} | ||
== | ==Visualization of protein-protein interactions in ''B. subtilis''== | ||
* the beta version of an [http://cellpublisher.gobics.de/subtinteract/interactionList/PtsH interactive protein-protein interaction map] (just replace "PtsH" in the browser line by the protein of your interest) | * the beta version of an [http://cellpublisher.gobics.de/subtinteract/interactionList/PtsH interactive protein-protein interaction map] (just replace "PtsH" in the browser line by the protein of your interest) | ||
* the beta version of [http://subtiwiki.uni-goettingen.de/pathways/SubtInteract/demo_mitlinks/ SubtInteract] | * the beta version of [http://subtiwiki.uni-goettingen.de/pathways/SubtInteract/demo_mitlinks/ SubtInteract] | ||
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* [[transcription]]: [[RNA polymerase]] | * [[transcription]]: [[RNA polymerase]] | ||
* [[translation]]: the [[ribosomal proteins|ribosome]] | * [[translation]]: the [[ribosomal proteins|ribosome]] | ||
* [[RNases|RNA processing and degradation]]: the [[RNA degradosome]] | |||
* [[cell division]]: the [[divisome]] | * [[cell division]]: the [[divisome]] | ||
Revision as of 06:45, 4 January 2011
Protein-protein interactions are essential for many activities of any living cell. These interactions involve multi-protein complexes that take part in central processes such as DNA replication, transcription or translation. Protein-protein interactions may also be involved in a variety of regulatory events. Metabolic enzymes do often form transien complexes that represent a complete pathways. These complexes are called metabolon. Finally, many interactions may be of a transient nature.
Methods to detect protein-protein interactions
- Yeast Two Hybrid System PubMed
- TAP-Tag purification PubMed
Attention: Each technique detects only about 33% of all interactions PubMed
Visualization of protein-protein interactions in B. subtilis
- the beta version of an interactive protein-protein interaction map (just replace "PtsH" in the browser line by the protein of your interest)
- the beta version of SubtInteract
Protein complexes in B. subtilis
- DNA replication: the replisome
- transcription: RNA polymerase
- translation: the ribosome
- RNA processing and degradation: the RNA degradosome
- cell division: the divisome
- metabolism: the metabolons of glycolysis and the TCA cycle PubMed
Important publications
<pubmed>20658969 18228443 18219467 17953394 15264234 14706816 12127457 11827824 11377797 11306254 10851163 10842303 18366733 10694888 2200717 2441660 </pubmed>