YodU

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  • Description: N-terminal part of the split gene spsM (together with ypqP), spore envelope polysaccharide biosynthesis, in B. subtilis 168 the gene is disrupted by the SP-beta prophage

Gene name yodU
Synonyms ypqP, spsM
Essential no
Product unknown
Function spore envelope polysaccharide biosynthesis
Gene expression levels in SubtiExpress: yodU
MW, pI 15 kDa, 9.819
Gene length, protein length 423 bp, 141 aa (SpsM: 341 aa)
Immediate neighbours phy, sprB
Gene sequence (+200bp) Protein sequence
Genetic context
YodU context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YodU expression.png















Categories containing this gene/protein

sporulation proteins

This gene is a member of the following regulons

SigK regulon, GerE regulon

The gene

Basic information

  • Locus tag: BSU19810

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

  • rearrangement (deletion of the SP-beta prophage and generation of the spsM gene) occurs during sporulation and in response to DNA damage to allow production of spore polysaccharide PubMed

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s): EpsC (for SpsM)

Extended information on the protein

  • Kinetic information:
  • Domains: SpsM: Polysacc_synt_2 domain (Pfam accession number, PF02719) in the 18–296-aa region PubMed
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Pilar Sanchez-Vizuete, Dominique Le Coq, Arnaud Bridier, Jean-Marie Herry, Stéphane Aymerich, Romain Briandet
Identification of ypqP as a New Bacillus subtilis biofilm determinant that mediates the protection of Staphylococcus aureus against antimicrobial agents in mixed-species communities.
Appl Environ Microbiol: 2015, 81(1);109-18
[PubMed:25326298] [WorldCat.org] [DOI] (I p)

Kimihiro Abe, Yuta Kawano, Keito Iwamoto, Kenji Arai, Yuki Maruyama, Patrick Eichenberger, Tsutomu Sato
Developmentally-regulated excision of the SPβ prophage reconstitutes a gene required for spore envelope maturation in Bacillus subtilis.
PLoS Genet: 2014, 10(10);e1004636
[PubMed:25299644] [WorldCat.org] [DOI] (I e)

Patrick Eichenberger, Masaya Fujita, Shane T Jensen, Erin M Conlon, David Z Rudner, Stephanie T Wang, Caitlin Ferguson, Koki Haga, Tsutomu Sato, Jun S Liu, Richard Losick
The program of gene transcription for a single differentiating cell type during sporulation in Bacillus subtilis.
PLoS Biol: 2004, 2(10);e328
[PubMed:15383836] [WorldCat.org] [DOI] (I p)