SacC

• Description: levanase
  
  

Gene name	sacC
Synonyms	sacL
Essential	no
Product	levanase
Function	degradation of levan to fructose
MW, pI	75 kDa, 6.789
Gene length, protein length	2031 bp, 677 aa
Immediate neighbours	yraA, levG
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag:

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Hydrolysis of terminal, non-reducing (2->1)- and (2->6)-linked beta-D-fructofuranose residues in fructans (according to Swiss-Prot)

• Protein family: glycosyl hydrolase 32 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• Swiss prot entry: P05656

• KEGG entry: BSU27030

• E.C. number: 3.2.1.80

Expression and regulation

• Operon: levD-levE-levF-levG-sacC PubMed

• Sigma factor: SigL

• Regulation: repressed by glucose (CcpA) , carbon catabolite repression, induction by fructose

• Regulatory mechanism: CcpA: transcription repression, catabolite repression: transcription repression by CcpA, transcription activator LevR is less active in the presence of glucose; induction: transcription activation by LevR

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

1. Martin, I., Débarbouillé, M., Ferrari, E., Klier, A., and Rapoport, G. (1987) Characterization of the levanase gene of Bacillus subtilis which shows extensive homology to yeast invertase. Mol Gen Genet 208: 177-184. PubMed
2. Martin-Verstraete, I., Débarbouillé, M., Klier, A., and Rapoport, G. (1990) Levanase operon of Bacillus subtilis includes a fructose-specific phosphotransferase system regulating the expression of the operon. J Mol Biol 214: 657-671. PubMed
3. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed