levG

168

fructose-specific permease of the phosphotransferase system, EIID of the PTS

Locus: BSU_27040

Molecular weight: 29.94 kDa

Isoelectric point: 9.94

Protein length: 275 aa

Gene length: 828 bp

Function: fructose uptake and phosphorylation

Product: fructose-specific fructose-specific PTS, EIID component

Essential: no

Synonyms: levG

Genomic Context

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List of homologs in different organisms, belongs to COG3716 (Galperin et al., 2021)

Gene

Coordinates: 2,760,233 → 2,761,060

The protein

Protein family

• PTS permease, mannose family PubMed

Domains

• PTS EIID domain (aa 5-274) (according to UniProt)

Structure

• P26382 (AlphaFold)

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: levD-levE-levF-levG-sacC

  Description: PubMed

  Regulation

  • induced in the presence of fructose (LevR) PubMed

  Regulatory mechanism

  • CcpA: repression, PubMed, in ccpA regulon
  • LevR: activation, PubMed, in levR regulon

  Sigma factors

  • SigL: sigma factor, PubMed, in sigL regulon

Biological materials

Mutant

• BKE27040 (ΔlevG::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTTTCTTCCCCCTCAT,  downstream forward: _UP4_TAAGGTGTGTGAAGGAAAGA
• BKK27040 (ΔlevG::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTTTCTTCCCCCTCAT,  downstream forward: _UP4_TAAGGTGTGTGAAGGAAAGA

References

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