YodU

• Description: N-terminal part of the split gene spsM (together with ypqP), spore envelope polysaccharide biosynthesis, in B. subtilis 168 the gene is disrupted by the SP-beta prophage
  
  

Gene name	yodU
Synonyms	ypqP, spsM
Essential	no
Product	unknown
Function	spore envelope polysaccharide biosynthesis
Gene expression levels in SubtiExpress: yodU
MW, pI	15 kDa, 9.819
Gene length, protein length	423 bp, 141 aa (SpsM: 341 aa)
Immediate neighbours	phy, sprB
Gene sequence (+200bp)	Protein sequence
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

sporulation proteins

This gene is a member of the following regulons

SigK regulon, GerE regulon

The gene

Basic information

• Locus tag: BSU19810

Database entries

• BsubCyc: BSU19810

• DBTBS entry: no entry

• SubtiList entry: [1]

Additional information

• rearrangement (deletion of the SP-beta prophage and generation of the spsM gene) occurs during sporulation and in response to DNA damage to allow production of spore polysaccharide PubMed

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s): EpsC (for SpsM)

Extended information on the protein

• Kinetic information:

• Domains: SpsM: Polysacc_synt_2 domain (Pfam accession number, PF02719) in the 18–296-aa region PubMed

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • secreted (according to Swiss-Prot)

Database entries

• BsubCyc: BSU19810

• Structure:

• UniProt: O34490

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: yodU PubMed

• Sigma factor: SigK PubMed

• Regulation:
  • expressed late during sporulation in the mother cell (SigK, GerE) PubMed

• Regulatory mechanism:
  • GerE: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Pilar Sanchez-Vizuete, Dominique Le Coq, Arnaud Bridier, Jean-Marie Herry, Stéphane Aymerich, Romain Briandet
Identification of ypqP as a New Bacillus subtilis biofilm determinant that mediates the protection of Staphylococcus aureus against antimicrobial agents in mixed-species communities.
Appl Environ Microbiol: 2015, 81(1);109-18
[PubMed:25326298] [WorldCat.org] [DOI] (I p)

Kimihiro Abe, Yuta Kawano, Keito Iwamoto, Kenji Arai, Yuki Maruyama, Patrick Eichenberger, Tsutomu Sato
Developmentally-regulated excision of the SPβ prophage reconstitutes a gene required for spore envelope maturation in Bacillus subtilis.
PLoS Genet: 2014, 10(10);e1004636
[PubMed:25299644] [WorldCat.org] [DOI] (I e)

Patrick Eichenberger, Masaya Fujita, Shane T Jensen, Erin M Conlon, David Z Rudner, Stephanie T Wang, Caitlin Ferguson, Koki Haga, Tsutomu Sato, Jun S Liu, Richard Losick
The program of gene transcription for a single differentiating cell type during sporulation in Bacillus subtilis.
PLoS Biol: 2004, 2(10);e328
[PubMed:15383836] [WorldCat.org] [DOI] (I p)