BkdR

• Description: transcriptional activator of the ptb-bcd-buk-lpdV-bkdAA-bkdAB-bkdB operon
  
  

Gene name	bkdR
Synonyms	yqiR
Essential	no
Product	transcriptional activator (for SigL-dependent promoter))
Function	regulation of branched-chain amino acid utilization, required for survival at low temperatures)
Gene expression levels in SubtiExpress: bkdR required for survival at low temperatures)
Interactions involving this protein in SubtInteract: BkdR
Metabolic function and regulation of this protein in SubtiPathways: bkdR
MW, pI	77 kDa, 6.237
Gene length, protein length	2076 bp, 692 aa
Immediate neighbours	ptb, yqzF
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of amino acids, transcription factors and their control

This gene is a member of the following regulons

CodY regulon

The BkdR regulon:

• ptb-bcd-buk-lpdV-bkdAA-bkdAB-bkdB

The gene

Basic information

• Locus tag: BSU24100

Phenotypes of a mutant

The mutant is cold-sensitive PubMed

Database entries

• BsubCyc: BSU24100

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:
  • PAS domain

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: BkdR-SigL (activation)

• Localization:

Database entries

• BsubCyc: BSU24100

• Structure:

• UniProt: P54529

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: bkdR PubMed

• Expression browser: bkdR PubMed

• Sigma factor:

• Regulation:
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed

• Regulatory mechanism:
  • CodY: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:
  • 1A913 ( bkdR::kan), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Bjorn A Traag, Antonia Pugliese, Jonathan A Eisen, Richard Losick
Gene conservation among endospore-forming bacteria reveals additional sporulation genes in Bacillus subtilis.
J Bacteriol: 2013, 195(2);253-60
[PubMed:23123912] [WorldCat.org] [DOI] (I p)

Frank Wiegeshoff, Carsten L Beckering, Michel Debarbouille, Mohamed A Marahiel
Sigma L is important for cold shock adaptation of Bacillus subtilis.
J Bacteriol: 2006, 188(8);3130-3
[PubMed:16585774] [WorldCat.org] [DOI] (P p)

Ken-ichi Yoshida, Hirotake Yamaguchi, Masaki Kinehara, Yo-hei Ohki, Yoshiko Nakaura, Yasutaro Fujita
Identification of additional TnrA-regulated genes of Bacillus subtilis associated with a TnrA box.
Mol Microbiol: 2003, 49(1);157-65
[PubMed:12823818] [WorldCat.org] [DOI] (P p)

M Debarbouille, R Gardan, M Arnaud, G Rapoport
Role of bkdR, a transcriptional activator of the sigL-dependent isoleucine and valine degradation pathway in Bacillus subtilis.
J Bacteriol: 1999, 181(7);2059-66
[PubMed:10094682] [WorldCat.org] [DOI] (P p)