GltX
- Description: glutamyl-tRNA synthetase, universally conserved protein
| Gene name | gltX |
| Synonyms | |
| Essential | yes PubMed |
| Product | glutamyl-tRNA synthetase |
| Function | translation |
| Gene expression levels in SubtiExpress: gltX | |
| Interactions involving this protein in SubtInteract: GltX | |
| Metabolic function and regulation of this protein in SubtiPathways: gltX | |
| MW, pI | 55 kDa, 5.021 |
| Gene length, protein length | 1449 bp, 483 aa |
| Immediate neighbours | ispF, cysE |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
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Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
translation, essential genes, universally conserved proteins, most abundant proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU00920
Phenotypes of a mutant
essential PubMed
Database entries
- BsubCyc: BSU00920
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu) (according to Swiss-Prot)
- Protein family: class-I aminoacyl-tRNA synthetase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Modification:
- Effectors of protein activity:
- Interactions:
- (GatA-GatB-GatC)-GltX-trnS-Gln (the "transamidosome") PubMed, see a model of the interaction PubMed
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- BsubCyc: BSU00920
- Structure: 3AKZ (complex with tRNA-Gln from Thermotoga maritima, 38% identity, 62% similarity) PubMed
- UniProt: P22250
- KEGG entry: [3]
- E.C. number: 6.1.1.17
Additional information
Expression and regulation
- Regulation:
- expression transiently increases in the forespore PubMed
- Regulatory mechanism:
- Additional information:
- the mRNA is very stable (half-life > 15 min) PubMed
- belongs to the 100 most abundant proteins PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium): 1736 PubMed
- number of protein molecules per cell (complex medium with amino acids, without glucose): 5280 PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Alex Rosenberg, Lior Sinai, Yoav Smith, Sigal Ben-Yehuda
Dynamic expression of the translational machinery during Bacillus subtilis life cycle at a single cell level.
PLoS One: 2012, 7(7);e41921
[PubMed:22848659]
[WorldCat.org]
[DOI]
(I p)
Takuhiro Ito, Shigeyuki Yokoyama
Two enzymes bound to one transfer RNA assume alternative conformations for consecutive reactions.
Nature: 2010, 467(7315);612-6
[PubMed:20882017]
[WorldCat.org]
[DOI]
(I p)
Christine Eymann, Annette Dreisbach, Dirk Albrecht, Jörg Bernhardt, Dörte Becher, Sandy Gentner, Le Thi Tam, Knut Büttner, Gerrit Buurman, Christian Scharf, Simone Venz, Uwe Völker, Michael Hecker
A comprehensive proteome map of growing Bacillus subtilis cells.
Proteomics: 2004, 4(10);2849-76
[PubMed:15378759]
[WorldCat.org]
[DOI]
(P p)
G Hambraeus, C von Wachenfeldt, L Hederstedt
Genome-wide survey of mRNA half-lives in Bacillus subtilis identifies extremely stable mRNAs.
Mol Genet Genomics: 2003, 269(5);706-14
[PubMed:12884008]
[WorldCat.org]
[DOI]
(P p)
