SpoIID

• Description: cell wall hydrolase (lytic transglycosylase), required for complete dissolution of the asymmetric septum
  
  

Gene name	spoIID
Synonyms	spoIIC
Essential	no
Product	lytic transglycosylase
Function	dissolution of the septal cell wall
Gene expression levels in SubtiExpress: spoIID
Interactions involving this protein in SubtInteract: SpoIID
MW, pI	37 kDa, 9.764
Gene length, protein length	1029 bp, 343 aa
Immediate neighbours	ywmC, murAA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall/ other, sporulation proteins, membrane proteins

This gene is a member of the following regulons

SigE regulon, SpoIIID regulon

The gene

Basic information

• Locus tag: BSU36750

Database entries

• BsubCyc: BSU36750

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • degrades the glycan strands of the peptidoglycan into disaccharide units PubMed, enhances SpoIIP activity PubMed
  • required for proper localization of SpoIIQ (together with SpoIIP) PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: SpoIID-SpoIIP PubMed

• Localization: cell membrane PubMed

Database entries

• BsubCyc: BSU36750

• Structure:

• UniProt: P07372

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: spoIID PubMed

• Expression browser: spoIID PubMed

• Sigma factor: SigE PubMed

• Regulation:
  • expressed early during sporulation in the mother cell (SigE, SpoIIID) PubMed

• Regulatory mechanism:
  • SpoIIID: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Lotte Søgaard-Andersen
Stably bridging a great divide: localization of the SpoIIQ landmark protein in Bacillus subtilis.
Mol Microbiol: 2013, 89(6);1019-24
[PubMed:23944268] [WorldCat.org] [DOI] (I p)

Original publications

Jennifer Fredlund, Dan Broder, Tinya Fleming, Clémence Claussin, Kit Pogliano
The SpoIIQ landmark protein has different requirements for septal localization and immobilization.
Mol Microbiol: 2013, 89(6);1053-68
[PubMed:23859254] [WorldCat.org] [DOI] (I p)

Christopher D A Rodrigues, Kathleen A Marquis, Jeffrey Meisner, David Z Rudner
Peptidoglycan hydrolysis is required for assembly and activity of the transenvelope secretion complex during sporulation in Bacillus subtilis.
Mol Microbiol: 2013, 89(6);1039-52
[PubMed:23834622] [WorldCat.org] [DOI] (I p)

Pablo Meyer, Jennifer Gutierrez, Kit Pogliano, Jonathan Dworkin
Cell wall synthesis is necessary for membrane dynamics during sporulation of Bacillus subtilis.
Mol Microbiol: 2010, 76(4);956-70
[PubMed:20444098] [WorldCat.org] [DOI] (I p)

Jennifer Gutierrez, Rachelle Smith, Kit Pogliano
SpoIID-mediated peptidoglycan degradation is required throughout engulfment during Bacillus subtilis sporulation.
J Bacteriol: 2010, 192(12);3174-86
[PubMed:20382772] [WorldCat.org] [DOI] (I p)

Cecile Morlot, Tsuyoshi Uehara, Kathleen A Marquis, Thomas G Bernhardt, David Z Rudner
A highly coordinated cell wall degradation machine governs spore morphogenesis in Bacillus subtilis.
Genes Dev: 2010, 24(4);411-22
[PubMed:20159959] [WorldCat.org] [DOI] (I p)

Arnaud Chastanet, Richard Losick
Engulfment during sporulation in Bacillus subtilis is governed by a multi-protein complex containing tandemly acting autolysins.
Mol Microbiol: 2007, 64(1);139-52
[PubMed:17376078] [WorldCat.org] [DOI] (P p)

Thierry Doan, Kathleen A Marquis, David Z Rudner
Subcellular localization of a sporulation membrane protein is achieved through a network of interactions along and across the septum.
Mol Microbiol: 2005, 55(6);1767-81
[PubMed:15752199] [WorldCat.org] [DOI] (P p)

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

Patrick Eichenberger, Masaya Fujita, Shane T Jensen, Erin M Conlon, David Z Rudner, Stephanie T Wang, Caitlin Ferguson, Koki Haga, Tsutomu Sato, Jun S Liu, Richard Losick
The program of gene transcription for a single differentiating cell type during sporulation in Bacillus subtilis.
PLoS Biol: 2004, 2(10);e328
[PubMed:15383836] [WorldCat.org] [DOI] (I p)

P Eichenberger, P Fawcett, R Losick
A three-protein inhibitor of polar septation during sporulation in Bacillus subtilis.
Mol Microbiol: 2001, 42(5);1147-62
[PubMed:11886548] [WorldCat.org] [DOI] (P p)

A Driks, R Losick
Compartmentalized expression of a gene under the control of sporulation transcription factor sigma E in Bacillus subtilis.
Proc Natl Acad Sci U S A: 1991, 88(22);9934-8
[PubMed:1946462] [WorldCat.org] [DOI] (P p)

I Lopez-Diaz, S Clarke, J Mandelstam
spoIID operon of Bacillus subtilis: cloning and sequence.
J Gen Microbiol: 1986, 132(2);341-54
[PubMed:3011962] [WorldCat.org] [DOI] (P p)