SacC

• Description: levanase
  
  

Gene name	sacC
Synonyms
Essential	no
Product	levanase
Function	degradation of levan to fructose
Gene expression levels in SubtiExpress: sacC
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	75 kDa, 6.789
Gene length, protein length	2031 bp, 677 aa
Immediate neighbours	yraA, levG
Sequences	Protein DNA Advanced_DNA
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

CcpA regulon, LevR regulon, SigL regulon

The gene

Basic information

• Locus tag: BSU27030

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Hydrolysis of terminal, non-reducing (2->1)- and (2->6)-linked beta-D-fructofuranose residues in fructans (according to Swiss-Prot)

• Protein family: glycosyl hydrolase 32 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P05656

• KEGG entry: [3]

• E.C. number: 3.2.1.80

Expression and regulation

• Operon: levD-levE-levF-levG-sacC PubMed

• Expression browser: sacC PubMed

• Sigma factor: SigL PubMed

• Regulation:
  • subject to carbon catabolite repression (CcpA, LevR) PubMed
  • induced in the presence of fructose (LevR) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed
  • LevR: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Massimiliano Marvasi, Pieter T Visscher, Lilliam Casillas Martinez
Exopolymeric substances (EPS) from Bacillus subtilis: polymers and genes encoding their synthesis.
FEMS Microbiol Lett: 2010, 313(1);1-9
[PubMed:20735481] [WorldCat.org] [DOI] (I p)

Original publications

Additional publications: PubMed