YqeV

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  • Description: tRNA methylthiotransferase

Gene name yqeV
Synonyms
Essential no
Product tRNA methylthiotransferase
Function tRNA maturation
MW, pI 51 kDa, 5.242
Gene length, protein length 1353 bp, 451 aa
Immediate neighbours yqeW, yqeU
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YqeV context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YqeV expression.png
























Categories containing this gene/protein

translation, heat shock proteins, membrane proteins

This gene is a member of the following regulons

HrcA regulon

The gene

Basic information

  • Locus tag: BSU25430

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: modification of N(6)-threonylcarbamoyladenosine (t(6)A) to 2-methylthio-N(6)-threonylcarbamoyladenosine (ms(2)t(6)A) in tRNA PubMed
  • Protein family: YqeV subfamily (according to Swiss-Prot)
  • Paralogous protein(s): YmcB

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s): 2 (4Fe-4S) clusters PubMed
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Simon Arragain, Samuel K Handelman, Farhad Forouhar, Fan-Yan Wei, Kazuhito Tomizawa, John F Hunt, Thierry Douki, Marc Fontecave, Etienne Mulliez, Mohamed Atta
Identification of eukaryotic and prokaryotic methylthiotransferase for biosynthesis of 2-methylthio-N6-threonylcarbamoyladenosine in tRNA.
J Biol Chem: 2010, 285(37);28425-33
[PubMed:20584901] [WorldCat.org] [DOI] (I p)

Brian P Anton, Susan P Russell, Jason Vertrees, Simon Kasif, Elisabeth A Raleigh, Patrick A Limbach, Richard J Roberts
Functional characterization of the YmcB and YqeV tRNA methylthiotransferases of Bacillus subtilis.
Nucleic Acids Res: 2010, 38(18);6195-205
[PubMed:20472640] [WorldCat.org] [DOI] (I p)

G Homuth, A Mogk, W Schumann
Post-transcriptional regulation of the Bacillus subtilis dnaK operon.
Mol Microbiol: 1999, 32(6);1183-97
[PubMed:10383760] [WorldCat.org] [DOI] (P p)

G Homuth, S Masuda, A Mogk, Y Kobayashi, W Schumann
The dnaK operon of Bacillus subtilis is heptacistronic.
J Bacteriol: 1997, 179(4);1153-64
[PubMed:9023197] [WorldCat.org] [DOI] (P p)

G Yuan, S L Wong
Isolation and characterization of Bacillus subtilis groE regulatory mutants: evidence for orf39 in the dnaK operon as a repressor gene in regulating the expression of both groE and dnaK.
J Bacteriol: 1995, 177(22);6462-8
[PubMed:7592421] [WorldCat.org] [DOI] (P p)