dnaJ

168

heat-shock protein (activation of DnaK)

Locus: BSU_25460

Molecular weight: 40.69 kDa

Isoelectric point: 7.92

Protein length: 375 aa

Gene length: 1128 bp

Function: protein quality control

Product: heat-shock protein (activation of DnaK)

Essential: no

Synonyms: dnaJ

Genomic Context

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List of homologs in different organisms, belongs to COG0484 (Galperin et al., 2021)

Gene

Coordinates: 2,624,785 → 2,625,912

The protein

Protein family

• dnaJ family (single member, according to UniProt)

Domains

• J domain (aa 5-69) (according to UniProt)

Structure

• 3LZ8 (PDB) (from Klebsiella pneumoniae, 34% identity)
• P17631 (AlphaFold)

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: hrcA-grpE-dnaK-dnaJ-yqeT-yqeU-yqeV

  Description: PubMed

  Regulation

  • induced by heat shock (HrcA) PubMed

  Regulatory mechanism

  • HrcA: repression, PubMed, in hrcA regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• BKE25460 (ΔdnaJ::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTCGCTTCACTCTCCCG,  downstream forward: _UP4_TAATTATTGGATGAGGAGTT
• BKK25460 (ΔdnaJ::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTCGCTTCACTCTCCCG,  downstream forward: _UP4_TAATTATTGGATGAGGAGTT

Labs working on this gene/protein

• Wolfgang Schumann, Bayreuth University, Germany Homepage

References

Reviews

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Original Publications

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