yqeU

168

ribosomal RNA small subunit methyltransferase E

Locus: BSU_25440

Molecular weight: 28.65 kDa

Isoelectric point: 5.29

Protein length: 256 aa

Gene length: 771 bp

Function: rRNA maturation

Product: ribosomal RNA small subunit methyltransferase E

Essential: no

Synonyms: yqeU, rsmE

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG1385 (Galperin et al., 2021)

Gene

Coordinates: 2,623,032 → 2,623,802

The protein

Catalyzed reaction/ biological activity

• S-adenosyl-L-methionine + uridine¹⁴⁹⁸ in 16S rRNA --> H⁺ + N³-methyluridine¹⁴⁹⁸ in 16S rRNA + S-adenosyl-L-homocysteine (according to UniProt)

Protein family

• RNA methyltransferase rsmE family (single member, according to UniProt)

Structure

• 1VHK (PDB) PubMed
• P54461 (AlphaFold)

Expression and Regulation

Operons

• Genes: hrcA-grpE-dnaK-dnaJ-yqeT-yqeU-yqeV

  Description: PubMed

  Regulation

  • induced by heat shock (HrcA) PubMed

  Regulatory mechanism

  • HrcA: repression, PubMed, in hrcA regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Additional information

• the mRNA is cleaved by RNase III PubMed

Biological materials

Mutant

• MGNA-C497 (yqeU::erm), available at the NBRP B. subtilis, Japan
• BKE25440 (ΔyqeU::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATACGCTGTGACACCTACT,  downstream forward: _UP4_GAGTTATTAAGAGGTGATCA
• BKK25440 (ΔyqeU::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATACGCTGTGACACCTACT,  downstream forward: _UP4_GAGTTATTAAGAGGTGATCA

References

Loading