YqeV

• Description: tRNA methylthiotransferase
  
  

Gene name	yqeV
Synonyms
Essential	no
Product	tRNA methylthiotransferase
Function	tRNA maturation
MW, pI	51 kDa, 5.242
Gene length, protein length	1353 bp, 451 aa
Immediate neighbours	yqeW, yqeU
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

translation, heat shock proteins, membrane proteins

This gene is a member of the following regulons

HrcA regulon

The gene

Basic information

• Locus tag: BSU25430

Expression

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: modification of N(6)-threonylcarbamoyladenosine (t(6)A) to 2-methylthio-N(6)-threonylcarbamoyladenosine (ms(2)t(6)A) in tRNA PubMed

• Protein family: YqeV subfamily (according to Swiss-Prot)

• Paralogous protein(s): YmcB

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s): 2 (4Fe-4S) clusters PubMed

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P54462

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: hrcA-grpE-dnaK-dnaJ-yqeT-yqeU-yqeV PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by heat shock (HrcA) PubMed

• Regulatory mechanism:
  • HrcA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Simon Arragain, Samuel K Handelman, Farhad Forouhar, Fan-Yan Wei, Kazuhito Tomizawa, John F Hunt, Thierry Douki, Marc Fontecave, Etienne Mulliez, Mohamed Atta
Identification of eukaryotic and prokaryotic methylthiotransferase for biosynthesis of 2-methylthio-N6-threonylcarbamoyladenosine in tRNA.
J Biol Chem: 2010, 285(37);28425-33
[PubMed:20584901] [WorldCat.org] [DOI] (I p)

Brian P Anton, Susan P Russell, Jason Vertrees, Simon Kasif, Elisabeth A Raleigh, Patrick A Limbach, Richard J Roberts
Functional characterization of the YmcB and YqeV tRNA methylthiotransferases of Bacillus subtilis.
Nucleic Acids Res: 2010, 38(18);6195-205
[PubMed:20472640] [WorldCat.org] [DOI] (I p)

G Homuth, A Mogk, W Schumann
Post-transcriptional regulation of the Bacillus subtilis dnaK operon.
Mol Microbiol: 1999, 32(6);1183-97
[PubMed:10383760] [WorldCat.org] [DOI] (P p)

G Homuth, S Masuda, A Mogk, Y Kobayashi, W Schumann
The dnaK operon of Bacillus subtilis is heptacistronic.
J Bacteriol: 1997, 179(4);1153-64
[PubMed:9023197] [WorldCat.org] [DOI] (P p)

G Yuan, S L Wong
Isolation and characterization of Bacillus subtilis groE regulatory mutants: evidence for orf39 in the dnaK operon as a repressor gene in regulating the expression of both groE and dnaK.
J Bacteriol: 1995, 177(22);6462-8
[PubMed:7592421] [WorldCat.org] [DOI] (P p)