SpoIIR

• Description: required for processing and compartmentalization of pro-SigE
  
  

Gene name	spoIIR
Synonyms	ipc-27d, csfX
Essential	no
Product	morphological chekcpoint for SigE processing
Function	control of SigE activation
MW, pI	25 kDa, 4.737
Gene length, protein length	672 bp, 224 aa
Immediate neighbours	ywlB, ywlA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

sigma factors and their control, sporulation proteins

This gene is a member of the following regulons

SigF regulon

The gene

Basic information

• Locus tag: BSU36970

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: interacts with and activates the pro-SigE protease SpoIIGA in the intermembrane space between the forespore and the mother cell PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • likely acylated on Thr-27 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • SpoIIR-SpoIIGA PubMed

• Localization:
  • localizes initially to the forespore septal membrane (in a SpoIIGA-dependent manner), is then exported to the intermembrane space between the forespore and the mother cell PubMed

Database entries

• Structure:

• UniProt: P39151

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: spoIIR (according to DBTBS)

• Sigma factor: SigF PubMed

• Regulation:
  • expressed early during sporulation in the forespore (SigF) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed

Vasant K Chary, Panagiotis Xenopoulos, Avigdor Eldar, Patrick J Piggot
Loss of compartmentalization of σ(E) activity need not prevent formation of spores by Bacillus subtilis.
J Bacteriol: 2010, 192(21);5616-24
[PubMed:20802044] [WorldCat.org] [DOI] (I p)

Avigdor Eldar, Vasant K Chary, Panagiotis Xenopoulos, Michelle E Fontes, Oliver C Losón, Jonathan Dworkin, Patrick J Piggot, Michael B Elowitz
Partial penetrance facilitates developmental evolution in bacteria.
Nature: 2009, 460(7254);510-4
[PubMed:19578359] [WorldCat.org] [DOI] (I p)

Stephanie T Wang, Barbara Setlow, Erin M Conlon, Jessica L Lyon, Daisuke Imamura, Tsutomu Sato, Peter Setlow, Richard Losick, Patrick Eichenberger
The forespore line of gene expression in Bacillus subtilis.
J Mol Biol: 2006, 358(1);16-37
[PubMed:16497325] [WorldCat.org] [DOI] (P p)

Aileen Rubio, Kit Pogliano
Septal localization of forespore membrane proteins during engulfment in Bacillus subtilis.
EMBO J: 2004, 23(7);1636-46
[PubMed:15044948] [WorldCat.org] [DOI] (P p)

Masaya Fujita, Richard Losick
An investigation into the compartmentalization of the sporulation transcription factor sigmaE in Bacillus subtilis.
Mol Microbiol: 2002, 43(1);27-38
[PubMed:11849534] [WorldCat.org] [DOI] (P p)

A E Hofmeister, A Londoño-Vallejo, E Harry, P Stragier, R Losick
Extracellular signal protein triggering the proteolytic activation of a developmental transcription factor in B. subtilis.
Cell: 1995, 83(2);219-26
[PubMed:7585939] [WorldCat.org] [DOI] (P p)