spoIIR

168

required for processing and compartmentalization of pro-SigE

Locus: BSU_36970

Molecular weight: 25.20 kDa

Isoelectric point: 4.74

Protein length: 224 aa

Gene length: 675 bp

Function: control of SigE activation

Product: morphological chekcpoint for SigE processing

Essential: no

Synonyms: spoIIR, ipc-27d, csfX

Genomic Context

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List of homologs in different organisms, belongs to COG5822 (Galperin et al., 2021)

Gene

Coordinates: 3,794,676  3,795,350

The protein

Catalyzed reaction/ biological activity

• interacts with and activates the pro-SigE protease SpoIIGA in the intermembrane space between the forespore and the mother cell PubMed

Structure

• P39151 (AlphaFold)

Modification

• likely acylated on Thr-27 PubMed

Localization

• localizes initially to the forespore septal membrane (in a SpoIIGA-dependent manner), is then exported to the intermembrane space between the forespore and the mother cell PubMed

Expression and Regulation

Operons

• Genes: spoIIR

  Description: (according to DBTBS) null

  Regulation

  • expressed early during sporulation in the forespore (SigF) PubMed

  Sigma factors

  • SigF: sigma factor, PubMed, in sigF regulon

Biological materials

Mutant

• BKE36970 (spoIIR::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCGGTCCCCACCGTTCCT,  downstream forward: _UP4_TAATCATCTGGTCTAAAAAT
• BKK36970 (spoIIR::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCGGTCCCCACCGTTCCT,  downstream forward: _UP4_TAATCATCTGGTCTAAAAAT

References

Reviews

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Original Publications

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