spoIIGA

168

Pro-SigE protease

Locus: BSU_15310

Molecular weight: 34.70 kDa

Isoelectric point: 8.55

Protein length: 309 aa

Gene length: 930 bp

Function: maturation of SigE

Product: Pro-SigE protease

Essential: no

Synonyms: spoIIGA

Genomic Context

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List of homologs in different organisms, belongs to COG5818 (Galperin et al., 2021)

Gene

Coordinates: 1,603,779  1,604,708

The protein

Protein family

• peptidase U4 family (single member, according to UniProt)

Structure

• P13801 (AlphaFold)

Effectors of protein activity

• SpoIIGA protease activity is stimulated by the interaction with SpoIIR in the intermembrane space between the forespore and the mother cell PubMed

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: spoIIGA-sigE-sigG

  Description: PubMed

  Regulation

  • ''spoIIGA'': expressed under conditions that trigger sporulation (Spo0A) PubMed

  Regulatory mechanism

  • SinR: repression, in sinR regulon
  • Spo0A: activation, PubMed, in spo0A regulon
  • AbrB: repression, PubMed, in abrB regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon
  • SigG: sigma factor, PubMed, in sigG regulon
  • SigF: sigma factor, in sigF regulon

  Additional information

  • the mRNA half-life is about 2.6 min PubMed

Biological materials

Mutant

• BKE15310 (spoIIGA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACATCTGACTCCTTTCTTT,  downstream forward: _UP4_TAATGTTCGCAAATGTCCGT
• BKK15310 (spoIIGA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACATCTGACTCCTTTCTTT,  downstream forward: _UP4_TAATGTTCGCAAATGTCCGT

References

Reviews

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Original Publications

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