Spo0A

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• Description: phosphorelay regulator, initiation of sporulation <br/><br/>

Gene name	spo0A
Synonyms	spo0C, spo0G, spoIIL, sof-1
Essential	no
Product	phosphorelay response regulator
Function	initiation of sporulation
Metabolic function and regulation of this protein in SubtiPathways: <br/>Biofilm, Nucleotides (regulation), Ammonium/ glutamate, <br/>Central C-metabolism,Sugar catabolism, Phosphorelay, Stress, <br/>tRNA charging,Lipid synthesis
MW, pI	29 kDa, 5.989
Gene length, protein length	801 bp, 267 aa
Immediate neighbours	yqiG, spoIVB
Get the DNA and protein sequences <br/> (Barbe et al., 2009)
Genetic context <br/> <div align="right"> <small>This image was kindly provided by SubtiList</small></div>

<br/><br/><br/><br/><br/><br/>

The gene

Basic information

• Locus tag: BSU24220

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Genes controlled by Spo0A

• Activation by Spo0A: spoIIAA-spoIIAB-sigF, spoIIE, spoIIGA-sigE-sigG

• Repression by Spo0A: abrB, kinA, kinC, spo0A, metS

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: receives phosphorylation from Spo0B, dephosphorylation by Spo0E, direct phosphorylation by KinC upon potassium leakage PubMed

• Cofactor(s):

• Effectors of protein activity: the phosphorylation state affects DNA binding activity

• Interactions: KinC-Spo0A PubMed, Spo0A-Spo0E PubMed, Spo0A-SigA PubMed, Spo0A-Spo0B

• Localization:

Database entries

• Structure: 1QMP (with phosphorylated aspartate, Geobacillus stearothermophilus), 1FC3 (trans-activation domain, Geobacillus stearothermophilus), 1LQ1 (complex with DNA)

• UniProt: P06534

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: spo0A PubMed

• Sigma factor: SigA PubMed, SigH PubMed

• Regulation:
  • repressed under conditions that trigger sporulation (Spo0A) PubMed
  • repressed by casamino acids PubMed

• Regulatory mechanism:
  • Spo0A: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

• Tony Wilkinson, York University, U.K. homepage
• Imrich Barak, Slovak Academy of Science, Bratislava, Slovakia homepage
• Charles Moran, Emory University, NC, USA homepage

References

Reviews

<pubmed>19995980 20030732 20157337 11286862 20833318 20955377 </pubmed>

Original Publications

<pubmed>20413551 20404177 19528067, 19581368 , 8730857,2106683,3157992, 11254139,1569009, 12067336,10869437, 16385044,9287005, 8127878,9287022, 8231806, 1905258,16452424,2118505,8207022, 12176382, 9733708, 11069677,1391039,12730135, 2437099,8288522,16166384,9495766,2118512,1556084, 18296515,7768874, 3145384, 19114652 20689749 14651647,1537790,9658000, 8509330,14762002, 1846779, 2981817, 15687200, 9477965,8600030, 18840696, 1391039, 14679239, 14976210, 19207565 11679073 12270811 11112444 18978066 20154131 14712656 17157871 15060025 </pubmed>