spo0B

168

Sporulation initiation phosphotransferase of the phosphorelay

Locus: BSU_27930

Molecular weight: 22.40 kDa

Isoelectric point: 4.96

Protein length: 192 aa

Gene length: 579 bp

Function: initiation of sporulation

Product: sporulation initiation phosphotransferase

Essential: no

Synonyms: spo0B, spo0D

Genomic Context

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List of homologs in different organisms, belongs to COG5802 (Galperin et al., 2021)

Gene

Coordinates: 2,853,981  2,854,559

Phenotypes of a mutant

• Arrest of sporulation at stage 0 (initiation) PubMed

The protein

Structure

• 1F51 (PDB) (complex with Spo0F)
• 1IXM (PDB)
• P06535 (AlphaFold)

Expression and Regulation

Operons

• Genes: spo0B-obg-thrR-pheA

  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

  Additional information

  • expression is repressed by binding of RpoE to the A-rich sequence in the -35 region of the promoter PubMed

Biological materials

Mutant

• spo0B12, point mutation 85C>T (=R29W), available in BGSC 1S54
• spo0B136, amber mutation 103A>T (=K35X), available in BGSC 1S16
• spo0B580ts, point mutation 193G>A (=E65K), available in BGSC 1S90
• spo0B581ts, point mutation 461G>A (=G154D), available in BGSC 1S91
• BKE27930 (spo0B::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTCGCACTCCCAATCA,  downstream forward: _UP4_TAGCGGAGTTTTTAACGGTT
• BKK27930 (spo0B::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTCGCACTCCCAATCA,  downstream forward: _UP4_TAGCGGAGTTTTTAACGGTT

References

Reviews

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Original Publications

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