PfeT

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  • Description: Fe(II) efflux pump, P1B4 -type ATPase, protects the cell against iron intoxication

Gene name zosA
Synonyms ykvW
Essential no
Product Fe(II) efflux pump
Function protection against toxic iron
Gene expression levels in SubtiExpress: pfeT
Metabolic function and regulation of this protein in SubtiPathways:
PfeT
MW, pI 68 kDa, 4.934
Gene length, protein length 1911 bp, 637 aa
Immediate neighbours stoA, papB
Sequences Protein DNA DNA_with_flanks
Genetic context
YkvW context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
ZosA expression.png















Categories containing this gene/protein

transporters/ other, trace metal homeostasis (Cu, Zn, Ni, Mn, Mo), membrane proteins

This gene is a member of the following regulons

PerR regulon

The gene

Basic information

  • Locus tag: BSU13850

Phenotypes of a mutant

    • sensitivity to iron, this can be suppressed by low levels of Mn(II) PubMed
    • reduced genetic competence, can be rescued by the addition of excess zinc PubMed
    • reduced expression of comK, control is exerted at the post-transcriptional level and can be rescued by the addition of excess zinc PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + H2O + Zn2+(In) = ADP + phosphate + Zn2+(Out) (according to Swiss-Prot) P-type zinc-transporting ATPase PubMed
  • Protein family: Type IB subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Regulation: induced by hydrogen peroxide (PerR) PubMed
  • Regulatory mechanism:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Guohua Guan, Azul Pinochet-Barros, Ahmed Gaballa, Sarju J Patel, José M Argüello, John D Helmann
PfeT, a P1B4 -type ATPase, effluxes ferrous iron and protects Bacillus subtilis against iron intoxication.
Mol Microbiol: 2015, 98(4);787-803
[PubMed:26261021] [WorldCat.org] [DOI] (I p)

Mitsuo Ogura
ZnuABC and ZosA zinc transporters are differently involved in competence development in Bacillus subtilis.
J Biochem: 2011, 150(6);615-25
[PubMed:21813502] [WorldCat.org] [DOI] (I p)

Irnov Irnov, Cynthia M Sharma, Jörg Vogel, Wade C Winkler
Identification of regulatory RNAs in Bacillus subtilis.
Nucleic Acids Res: 2010, 38(19);6637-51
[PubMed:20525796] [WorldCat.org] [DOI] (I p)

Mayuree Fuangthong, John D Helmann
Recognition of DNA by three ferric uptake regulator (Fur) homologs in Bacillus subtilis.
J Bacteriol: 2003, 185(21);6348-57
[PubMed:14563870] [WorldCat.org] [DOI] (P p)

Ahmed Gaballa, John D Helmann
Bacillus subtilis CPx-type ATPases: characterization of Cd, Zn, Co and Cu efflux systems.
Biometals: 2003, 16(4);497-505
[PubMed:12779235] [WorldCat.org] [DOI] (P p)

John D Helmann, Ming Fang Winston Wu, Ahmed Gaballa, Phil A Kobel, Maud M Morshedi, Paul Fawcett, Chris Paddon
The global transcriptional response of Bacillus subtilis to peroxide stress is coordinated by three transcription factors.
J Bacteriol: 2003, 185(1);243-53
[PubMed:12486061] [WorldCat.org] [DOI] (P p)

Ahmed Gaballa, Tao Wang, Rick W Ye, John D Helmann
Functional analysis of the Bacillus subtilis Zur regulon.
J Bacteriol: 2002, 184(23);6508-14
[PubMed:12426338] [WorldCat.org] [DOI] (P p)

Ahmed Gaballa, John D Helmann
A peroxide-induced zinc uptake system plays an important role in protection against oxidative stress in Bacillus subtilis.
Mol Microbiol: 2002, 45(4);997-1005
[PubMed:12180919] [WorldCat.org] [DOI] (P p)