DprA

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  • Description: conveys incoming ssDNA to RecA; facilitates the displacement of both SSBs (SsbB and SsbA), increases RecA nucleation onto SSB-coated ssDNA, and mediates DNA strand annealing

Gene name dprA
Synonyms smf
Essential no
Product recombination mediator protein
Function DNA repair/ recombination
Gene expression levels in SubtiExpress: dprA
Interactions involving this protein in SubtInteract: DprA
MW, pI 32 kDa, 9.755
Gene length, protein length 891 bp, 297 aa
Immediate neighbours sucD, topA
Sequences Protein DNA DNA_with_flanks
Genetic context
Smf context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
DprA expression.png















Categories containing this gene/protein

DNA repair/ recombination, genetic competence

This gene is a member of the following regulons

ComK regulon

The gene

Basic information

  • Locus tag: BSU16110

Phenotypes of a mutant

    • strongly impaired genetic recombination PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • binds ssDNA, promotes displacement of SsbA and SsbB from ssDNA PubMed
    • provides RecA access to ssDNA during chromosomal transformation (together with RecO) PubMed
  • Protein family: smf family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Operon: dprA (according to DBTBS)
  • Sigma factor:
  • Regulation:
    • expressed under conditions that trigger genetic competence (ComK) PubMed
  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 57 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Peter Graumann, Freiburg University, Germany homepage

Your additional remarks

References