BioA

• Description: lysine-8-amino-7-oxononanoate aminotransferase
  
  

Gene name	bioA
Synonyms
Essential	no
Product	lysine-8-amino-7-oxononanoate aminotransferase
Function	biosynthesis of biotin
Gene expression levels in SubtiExpress: bioA
Metabolic function and regulation of this protein in SubtiPathways: bioA
MW, pI	49 kDa, 5.32
Gene length, protein length	1344 bp, 448 aa
Immediate neighbours	bioF, bioW
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis of cofactors

This gene is a member of the following regulons

BirA regulon

The gene

Basic information

• Locus tag: BSU30230

Database entries

• BsubCyc: BSU30230

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: class-III pyridoxal-phosphate-dependent aminotransferase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU30230

• Structure: 3DRD

• UniProt: P53555

• KEGG entry: [3]

• E.C. number: 2.6.1.62

Expression and regulation

• Operon: bioW-bioA-bioF-bioD-bioB-bioI-ytbQ PubMed

• Expression browser: bioA PubMed

• Sigma factor:

• Regulation:
  • repressed in the presence of biotin (BirA) PubMed

• Regulatory mechanism:
  • BirA: transcription repression PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 3192 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 3864 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 2260 PubMed

• • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 2738 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: