LpdV

• Description: 2-oxoisovalerate dehydrogenase (E3 subunit, dihydrolipoamide dehydrogenase)
  
  

Gene name	lpdV
Synonyms	yqiV, bkd
Essential	no
Product	2-oxoisovalerate dehydrogenase (E3 subunit, dihydrolipoamide dehydrogenase)
Function	utilization of branched-chain keto acids
Gene expression levels in SubtiExpress: lpdV
Metabolic function and regulation of this protein in SubtiPathways: lpdV
MW, pI	48 kDa, 4.893
Gene length, protein length	1371 bp, 457 aa
Immediate neighbours	bkdAA, buk
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of amino acids

This gene is a member of the following regulons

BkdR regulon, CodY regulon, SigL regulon

The gene

Basic information

• Locus tag: BSU24060

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Protein N(6)-(dihydrolipoyl)lysine + NAD⁺ = protein N(6)-(lipoyl)lysine + NADH (according to Swiss-Prot)

• Protein family: class-I pyridine nucleotide-disulfide oxidoreductase family (according to Swiss-Prot)

• Paralogous protein(s): AcoL, PdhD

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 2YQU (from Thermus thermophilus (hb8 mutant), 42% identity, 55% similarity)

• UniProt: P54533

• KEGG entry: [3]

• E.C. number: 1.8.1.4

Expression and regulation

• Operon: ptb-bcd-buk-lpdV-bkdAA-bkdAB-bkdB PubMed

• Expression browser: lpdV PubMed

• Sigma factor: SigL PubMed

• Regulation:
  • induced in the presence of isoleucine or valine (BkdR) PubMed

• Regulatory mechanism:
  • CodY: transcription repression PubMed
  • BkdR: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: