SpoIIR

• Description: required for processing and compartmentalization of pro-SigE
  
  

Gene name	spoIIR
Synonyms	ipc-27d, csfX
Essential	no
Product	morphological chekcpoint for SigE processing
Function	control of SigE activation
Gene expression levels in SubtiExpress: spoIIR
Interactions involving this protein in SubtInteract: SpoIIR
MW, pI	25 kDa, 4.737
Gene length, protein length	672 bp, 224 aa
Immediate neighbours	ywlB, ywlA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

sigma factors and their control, sporulation proteins

This gene is a member of the following regulons

SigF regulon

The gene

Basic information

• Locus tag: BSU36970

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: interacts with and activates the pro-SigE protease SpoIIGA in the intermembrane space between the forespore and the mother cell PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • likely acylated on Thr-27 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • SpoIIR-SpoIIGA PubMed

• Localization:
  • localizes initially to the forespore septal membrane (in a SpoIIGA-dependent manner), is then exported to the intermembrane space between the forespore and the mother cell PubMed

Database entries

• Structure:

• UniProt: P39151

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: spoIIR (according to DBTBS)

• Expression browser: spoIIR PubMed

• Sigma factor: SigF PubMed

• Regulation:
  • expressed early during sporulation in the forespore (SigF) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed

Avigdor Eldar, Vasant K Chary, Panagiotis Xenopoulos, Michelle E Fontes, Oliver C Losón, Jonathan Dworkin, Patrick J Piggot, Michael B Elowitz
Partial penetrance facilitates developmental evolution in bacteria.
Nature: 2009, 460(7254);510-4
[PubMed:19578359] [WorldCat.org] [DOI] (I p)

Stephanie T Wang, Barbara Setlow, Erin M Conlon, Jessica L Lyon, Daisuke Imamura, Tsutomu Sato, Peter Setlow, Richard Losick, Patrick Eichenberger
The forespore line of gene expression in Bacillus subtilis.
J Mol Biol: 2006, 358(1);16-37
[PubMed:16497325] [WorldCat.org] [DOI] (P p)

Aileen Rubio, Kit Pogliano
Septal localization of forespore membrane proteins during engulfment in Bacillus subtilis.
EMBO J: 2004, 23(7);1636-46
[PubMed:15044948] [WorldCat.org] [DOI] (P p)

Masaya Fujita, Richard Losick
An investigation into the compartmentalization of the sporulation transcription factor sigmaE in Bacillus subtilis.
Mol Microbiol: 2002, 43(1);27-38
[PubMed:11849534] [WorldCat.org] [DOI] (P p)

A E Hofmeister, A Londoño-Vallejo, E Harry, P Stragier, R Losick
Extracellular signal protein triggering the proteolytic activation of a developmental transcription factor in B. subtilis.
Cell: 1995, 83(2);219-26
[PubMed:7585939] [WorldCat.org] [DOI] (P p)