LevE

• Description: trigger enzyme: fructose-specific phosphotransferase system, EIIB component of the PTS
  
  

Gene name	levE
Synonyms
Essential	no
Product	trigger enzyme: fructose-specific phosphotransferase system, EIIB component
Function	fructose uptake and phosphorylation, control of LevR activity
Gene expression levels in SubtiExpress: levE
Interactions involving this protein in SubtInteract: LevE
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	17 kDa, 9.444
Gene length, protein length	486 bp, 162 aa
Immediate neighbours	levF, levD
Sequences	Protein DNA Advanced_DNA
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

phosphotransferase systems, utilization of specific carbon sources, transcription factors and their control, trigger enzyme, membrane proteins, phosphoproteins

This gene is a member of the following regulons

CcpA regulon, LevR regulon, SigL regulon

The gene

Basic information

• Locus tag: BSU27060

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Protein EIIB N(pi)-phospho-L-histidine + sugar = protein EIIB + sugar phosphate (according to Swiss-Prot)

• Protein family: PTS permease, mannose permease (Man) family PubMed

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: LevD-LevE, LevE-LevR

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure: 1BLE

• UniProt: P26380

• KEGG entry: [2]

• E.C. number: 2.7.1.69

Expression and regulation

• Operon: levD-levE-levF-levG-sacC PubMed

• Expression browser: levE PubMed

• Sigma factor: SigL PubMed

• Regulation:
  • subject to carbon catabolite repression (CcpA, LevR) PubMed
  • induced in the presence of fructose (LevR) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed
  • LevR: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: