MecA

• Description: buffering protein for development, dampens transitions to spore, biofilm exopolysaccharide and competence expression , targets ComK to ClpC-ClpP degradation machine (in log Phase), inhibits the transcriptional activity of Spo0A∼P by direct interaction
  
  

Gene name	mecA
Synonyms
Essential	no
Product	adaptor protein
Function	control of ComK degradation, regulation of competence
Gene expression levels in SubtiExpress: mecA
Interactions involving this protein in SubtInteract: MecA
MW, pI	25 kDa, 4.209
Gene length, protein length	654 bp, 218 aa
Immediate neighbours	yjbE, coiA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

genetic competence, proteolysis

The gene

Basic information

• Locus tag: BSU11520

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: mecA family (according to Swiss-Prot)

• Paralogous protein(s): YpbH

Extended information on the protein

• Kinetic information:

• Domains:
  • N-terminal domain (1 ... 120): recruitment of protein substrates PubMed
  • C-terminal domain (121 ... 218): facilitates assembly of the ClpC-ClpP oligomer and acts as a degradation tag PubMed

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • MecA-ComK PubMed
  • MecA-ComS PubMed
  • ClpC-MecA PubMed
  • MecA-Spo0A PubMed

• Localization:

Database entries

• Structure:
  • 3JTP (C-terminal domain) PubMed
  • 3PXG (the MecA-ClpC complex) PubMed

• UniProt: P37958

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: mecA PubMed

• Expression browser: mecA PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: GP813 (spc), GP814 (aphA3) both available in the Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Additional reviews: PubMed

Aurelia Battesti, Susan Gottesman
Roles of adaptor proteins in regulation of bacterial proteolysis.
Curr Opin Microbiol: 2013, 16(2);140-7
[PubMed:23375660] [WorldCat.org] [DOI] (I p)

Original Publications

Additonal publications: PubMed