BkdR

• Description: transcriptional activator of the ptb-bcd-buk-lpdV-bkdAA-bkdAB-bkdB operon
  
  

Gene name	bkdR
Synonyms	yqiR
Essential	no
Product	transcriptional activator (for SigL-dependent promoter))
Function	regulation of branched-chain amino acid utilization, required for survival at low temperatures)
Gene expression levels in SubtiExpress: bkdR required for survival at low temperatures)
Interactions involving this protein in SubtInteract: BkdR
Metabolic function and regulation of this protein in SubtiPathways: Lipid synthesis
MW, pI	77 kDa, 6.237
Gene length, protein length	2076 bp, 692 aa
Immediate neighbours	ptb, yqzF
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of amino acids, transcription factors and their control

This gene is a member of the following regulons

CodY regulon

The BkdR regulon:

• ptb-bcd-buk-lpdV-bkdAA-bkdAB-bkdB

The gene

Basic information

• Locus tag: BSU24100

Phenotypes of a mutant

The mutant is cold-sensitive PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: BkdR-SigL (activation)

• Localization:

Database entries

• Structure:

• UniProt: P54529

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: bkdR PubMed

• Expression browser: bkdR PubMed

• Sigma factor:

• Regulation:
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed

• Regulatory mechanism:
  • CodY: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:
  • 1A913 ( bkdR::kan), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Frank Wiegeshoff, Carsten L Beckering, Michel Debarbouille, Mohamed A Marahiel
Sigma L is important for cold shock adaptation of Bacillus subtilis.
J Bacteriol: 2006, 188(8);3130-3
[PubMed:16585774] [WorldCat.org] [DOI] (P p)

Ken-ichi Yoshida, Hirotake Yamaguchi, Masaki Kinehara, Yo-hei Ohki, Yoshiko Nakaura, Yasutaro Fujita
Identification of additional TnrA-regulated genes of Bacillus subtilis associated with a TnrA box.
Mol Microbiol: 2003, 49(1);157-65
[PubMed:12823818] [WorldCat.org] [DOI] (P p)

M Debarbouille, R Gardan, M Arnaud, G Rapoport
Role of bkdR, a transcriptional activator of the sigL-dependent isoleucine and valine degradation pathway in Bacillus subtilis.
J Bacteriol: 1999, 181(7);2059-66
[PubMed:10094682] [WorldCat.org] [DOI] (P p)