SpoIIR

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  • Description: required for processing and compartmentalization of pro-SigE

Gene name spoIIR
Synonyms ipc-27d, csfX
Essential no
Product morphological chekcpoint for SigE processing
Function control of SigE activation
Interactions involving this protein in SubtInteract: SpoIIR
MW, pI 25 kDa, 4.737
Gene length, protein length 672 bp, 224 aa
Immediate neighbours ywlB, ywlA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
SpoIIR context.gif
This image was kindly provided by SubtiList



Categories containing this gene/protein

sigma factors and their control, sporulation proteins

This gene is a member of the following regulons

SigF regulon

The gene

Basic information

  • Locus tag: BSU36970

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: interacts with and activates the pro-SigE protease SpoIIGA in the intermembrane space between the forespore and the mother cell PubMed
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
    • likely acylated on Thr-27 PubMed
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization:
    • localizes initially to the forespore septal membrane (in a SpoIIGA-dependent manner), is then exported to the intermembrane space between the forespore and the mother cell PubMed

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon: spoIIR (according to DBTBS)
  • Regulation:
    • expressed early during sporulation in the forespore (SigF) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Additional publications: PubMed

Avigdor Eldar, Vasant K Chary, Panagiotis Xenopoulos, Michelle E Fontes, Oliver C Losón, Jonathan Dworkin, Patrick J Piggot, Michael B Elowitz
Partial penetrance facilitates developmental evolution in bacteria.
Nature: 2009, 460(7254);510-4
[PubMed:19578359] [WorldCat.org] [DOI] (I p)

Stephanie T Wang, Barbara Setlow, Erin M Conlon, Jessica L Lyon, Daisuke Imamura, Tsutomu Sato, Peter Setlow, Richard Losick, Patrick Eichenberger
The forespore line of gene expression in Bacillus subtilis.
J Mol Biol: 2006, 358(1);16-37
[PubMed:16497325] [WorldCat.org] [DOI] (P p)

Aileen Rubio, Kit Pogliano
Septal localization of forespore membrane proteins during engulfment in Bacillus subtilis.
EMBO J: 2004, 23(7);1636-46
[PubMed:15044948] [WorldCat.org] [DOI] (P p)

Masaya Fujita, Richard Losick
An investigation into the compartmentalization of the sporulation transcription factor sigmaE in Bacillus subtilis.
Mol Microbiol: 2002, 43(1);27-38
[PubMed:11849534] [WorldCat.org] [DOI] (P p)

A E Hofmeister, A Londoño-Vallejo, E Harry, P Stragier, R Losick
Extracellular signal protein triggering the proteolytic activation of a developmental transcription factor in B. subtilis.
Cell: 1995, 83(2);219-26
[PubMed:7585939] [WorldCat.org] [DOI] (P p)