YqeV

• Description: tRNA methylthiotransferase
  
  

Gene name	yqeV
Synonyms
Essential	no
Product	tRNA methylthiotransferase
Function	tRNA maturation
MW, pI	51 kDa, 5.242
Gene length, protein length	1353 bp, 451 aa
Immediate neighbours	yqeW, yqeU
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU25430

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

translation, heat shock proteins, membrane proteins

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: modification of N(6)-threonylcarbamoyladenosine (t(6)A) to 2-methylthio-N(6)-threonylcarbamoyladenosine (ms(2)t(6)A) in tRNA PubMed

• Protein family: YqeV subfamily (according to Swiss-Prot)

• Paralogous protein(s): YmcB

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s): 2 (4Fe-4S) clusters PubMed

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P54462

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: hrcA-grpE-dnaK-dnaJ-yqeT-yqeU-yqeV PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by heat shock (HrcA) PubMed

• Regulatory mechanism:
  • HrcA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: