SacC

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  • Description: levanase

Gene name sacC
Synonyms sacL
Essential no
Product levanase
Function degradation of levan to fructose
MW, pI 75 kDa, 6.789
Gene length, protein length 2031 bp, 677 aa
Immediate neighbours yraA, levG
Gene sequence (+200bp) Protein sequence
Genetic context
SacC context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: repressed by glucose (CcpA) PubMed, carbon catabolite repression, induction by fructose
  • Regulatory mechanism: catabolite repression: transcription repression by CcpA, transcription activator LevR is less active in the presence of glucose; induction: transcription activation by LevR
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Blencke et al. (2003) Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways. Metab Eng. 5: 133-149 PubMed
  2. Martin, I., Débarbouillé, M., Ferrari, E., Klier, A., and Rapoport, G. (1987) Characterization of the levanase gene of Bacillus subtilis which shows extensive homology to yeast invertase. Mol Gen Genet 208: 177-184. PubMed
  3. Martin-Verstraete, I., Débarbouillé, M., Klier, A., and Rapoport, G. (1990) Levanase operon of Bacillus subtilis includes a fructose-specific phosphotransferase system regulating the expression of the operon. J Mol Biol 214: 657-671. PubMed
  4. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed